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Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB116
Plasmid#68570PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdToā¦ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB215
Plasmid#68606PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citriā¦ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Centrin3
Plasmid#136806PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin3
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
UseTagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SPromoterAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBa.Kif5c 1-559.GFP
Plasmid#45059DepositorInsertKif5c (Kif5c Rat)
UseTagsGFPExpressionMammalianMutationmotor domain aa1-559PromoterChicken Beta ActinAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk3a1
Plasmid#13758DepositorInsertJnk3a1 (MAPK10 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
HIV-dual-GT
Plasmid#122696PurposeDual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.DepositorInsertsmTagBFP2
DsRed-max
UseLentiviralTagsPEST domainExpressionMutationPromoterHIV-1 LTRAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-OPTN
Plasmid#175760PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTNDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-PGK-EiP
Plasmid#21312PurposeControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD4
Plasmid#20186DepositorInsertNotch 4 Intracellular Domain (Notch4 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 4 Intracellular Domain (Valā¦PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32481PurposeCre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swiā¦TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLOVE-Nmyc
Plasmid#15951DepositorInsertv-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quoā¦PromoterAvailable sinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
aav-SYN-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113760PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterSynapsinAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7AW)RS
Plasmid#207516PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2a1
Plasmid#13754DepositorInsertJnk2a1 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
LB901: pMVP/PB/Blast-DEST
Plasmid#121873PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vecā¦TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Cas9-gate
Plasmid#113743PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.DepositorInsertCas9
UseCRISPR; Gateway destination vectorTagsExpressionMutationPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFlag-CMV7-Erk2
Plasmid#39223DepositorInsertErk2 (Mapk1 Rat)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
LB701: pMVP/PB/mCherry-DEST
Plasmid#121871PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vecā¦TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM195A
Plasmid#218125PurposeHybrid circuit displaying hsCRBN-CTD bait and driving expression of gIIIDepositorInsertsCRBN_CTD
M13 pIII
UseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM194A
Plasmid#218124PurposeHybrid circuit displaying hsCRBN-CTD bait and driving expression of luxABDepositorInsertsCRBN_CTD
luxAB
UseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a2
Plasmid#13751DepositorInsertJnk1a2 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
Plasmid#51690PurposeYeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisGDepositorInserthisG-URA3-hisG (URA3 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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LC301: pMVP/PB/Zeo-DEST
Plasmid#121877PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vecā¦TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-CDO1
Plasmid#31292DepositorInsertcysteine dioxygenase, type I (CDO1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB114
Plasmid#68568PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)
Plasmid#107133PurposecGAL-N driver under the control of C. elegans hsp-16.41 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans hsp-16.41 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Brachyury-eGFP
Plasmid#21063DepositorInsertBrachyury promoter
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m3
Plasmid#149556PurposeGateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMsDepositorInsertecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domaiā¦PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFlag-CMV7-Erk2_KR
Plasmid#39224DepositorInsertErk2(K52R) (Mapk1 Rat)
UseTags3xFLAGExpressionMammalianMutationK52RPromoterCMVAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
IY601: pMVP/Lenti/Blast-DEST
Plasmid#121849PurposepMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9GFP-IRES-Neo
Plasmid#102907PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.DepositorInsertDDdCas9GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire-NRN
Plasmid#149692PurposeFor packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu_Cas9
Plasmid#216423PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-EGFP
Plasmid#100603PurposeDonor scaffold with EGFP positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsEGFP-T2A-Puro and tagBFPExpressionMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ172
Plasmid#98409PurposeDonor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsupstream homologous region to delete lys9 in genome by recombination
downstream homologous region to delete lys9 in genome by recombination
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only