Skip to main content
Addgene
Showing: 2241 - 2280 of 8751 results

  1. ER-J-TAG (pGS730)

    Plasmid
    #160038
    Purpose
    pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    3xHA-sfGFP-JPH-TMD
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160038-plasmid-map-sequence-id-319597

    Enlarge

  2. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73037
    Purpose
    Express Nluc and copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73037-plasmid-map-sequence-id-136876

    Enlarge

  3. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73040
    Purpose
    Express Nluc and copGFP dual reporter in mammalian cells
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK (phosphoglycerate kinase 1)
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73040-plasmid-map-sequence-id-136039

    Enlarge

  4. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73039
    Purpose
    Express Nluc, copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chicken beta-actin + CMV enhancer and chicken bet…
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73039-plasmid-map-sequence-id-136043

    Enlarge

  7. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

    Enlarge

  8. pJP72

    Plasmid
    #176479
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > GenR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176479-plasmid-map-sequence-id-346558

    Enlarge

  9. pJP102

    Plasmid
    #176480
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > NatR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176480-plasmid-map-sequence-id-346562

    Enlarge

  10. pJP103

    Plasmid
    #176481
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > HygR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176481-plasmid-map-sequence-id-346559

    Enlarge

  15. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1-a
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

    Enlarge

  16. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

    Enlarge

  17. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

    Enlarge

  19. pLentiCRISPR_mCherry

    Plasmid
    #229078
    Purpose
    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1s
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    229078-plasmid-map-sequence-id-456127

    Enlarge

  20. AV0-BWS-2.0

    Plasmid
    #124976
    Purpose
    Express gene of interest under EF1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124976-plasmid-map-sequence-id-243968

    Enlarge

  21. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    James Zhe Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

    Enlarge

  22. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

    Enlarge

  25. HSC1-D4Z4-GiP

    Plasmid
    #58542
    Purpose
    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58542-plasmid-map-sequence-id-90651

    Enlarge

  26. pLenti-EGFP-SnxA

    Plasmid
    #232460
    Purpose
    Lentiviral EGFP lipid sensor for PI(3,5)P2
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-SnxA
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232460-plasmid-map-sequence-id-464306

    Enlarge

  27. HSC1-HS4-D4Z4c-GiP

    Plasmid
    #58541
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58541-plasmid-map-sequence-id-90716

    Enlarge

  28. pWPI-myogenin

    Plasmid
    #79073
    Purpose
    Lentiviral plasmid that constitutively expresses human myogenin
    Depositor
    Jennifer Lei
    Insert
    human myogenin (MYOG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79073-plasmid-map-sequence-id-151242

    Enlarge

  29. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Expression
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

    Enlarge

  30. pLenti-EGFP-2xFYVE

    Plasmid
    #136996
    Purpose
    Lentiviral EGFP lipid sensor for PI(3)P
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-2xFYVE
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    136996-plasmid-map-sequence-id-270659

    Enlarge

  31. AV0-BWS-2.0-mC-G-CL3B

    Plasmid
    #124975
    Purpose
    evaluate autophagy
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-GFP-LC3B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1-alpha
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124975-plasmid-map-sequence-id-243955

    Enlarge

  32. pJG01

    Plasmid
    #213505
    Purpose
    expresses TdTomato in Capsaspora owczarzaki
    Depositor
    Joseph Gerdt
    Insert
    TdTomato
    Use
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213505-plasmid-map-sequence-id-431905

    Enlarge

  33. pLenti-EGFP-P4M-SidMx2

    Plasmid
    #136997
    Purpose
    Lentiviral EGFP lipid sensor for PI(4)P
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-P4M-SidMx2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136997-plasmid-map-sequence-id-291840

    Enlarge

  34. pLV_Zika_NS5_Flag

    Plasmid
    #79639
    Purpose
    Third generation Self-inactivating lentivector expressing NS5 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS5
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    79639-plasmid-map-sequence-id-148807

    Enlarge

  35. pLV_Zika_NS3_Flag

    Plasmid
    #79635
    Purpose
    Third generation Self-inactivating lentivector expressing NS3 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS3
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79635-plasmid-map-sequence-id-152697

    Enlarge

  36. pLV_Zika_NS2B_Flag

    Plasmid
    #79637
    Purpose
    Third generation Self-inactivating lentivector expressing NS2B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS2B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79637-plasmid-map-sequence-id-151178

    Enlarge

  37. pLV_Zika_Cv_Flag

    Plasmid
    #79628
    Purpose
    Third generation Self-inactivating lentivector expressing Cv from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    Cv
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79628-plasmid-map-sequence-id-148796

    Enlarge

  38. pLV_Zika_PrM_Flag

    Plasmid
    #79631
    Purpose
    Third generation Self-inactivating lentivector expressing PrM from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    PrM
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79631-plasmid-map-sequence-id-148799

    Enlarge

  39. pLV_Zika_NS4B_Flag

    Plasmid
    #79640
    Purpose
    Third generation Self-inactivating lentivector expressing NS4B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS4B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79640-plasmid-map-sequence-id-148808

    Enlarge

  40. pLV_Zika_Flag_NS4A

    Plasmid
    #79636
    Purpose
    Third generation Self-inactivating lentivector expressing NS4A from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS4A
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79636-plasmid-map-sequence-id-148804

    Enlarge

Showing: 2241 - 2280 of 8751 results