We narrowed to 11,866 results for: KIN

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pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV ArcLightCo (Q239)-T2A-nls-mCherry

Plasmid

#85806

Purpose

Genetically encoded voltage sensor ArcLight- codon optimized

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

ArcLightCo-Q239

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)Luc

Plasmid

#200112

Purpose

Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence in minimal p…

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Oct4-Cr1

Plasmid

#66989

Purpose

Expresses gRNA that targets stop codon for OCT4

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

gRNA for Crispr targeting the OCT4 stop codon

Expression

Mammalian

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-K179M

Plasmid

#49188

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

K179M

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-SLAMF6

Plasmid

#23396

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SLAMF6 (SLAMF6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

Plasmid

#90010

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C WT
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

Plasmid

#90013

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-Dicer

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PCK2

Plasmid

#20559

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PCK2 (PCK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PLK2

Plasmid

#20590

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK2 (PLK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag BMX

Plasmid

#20431

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BMX (BMX Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 PBD wt (Nigg AH12)

Plasmid

#41162

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD (PLK1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603)

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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A3A-PBE

Plasmid

#119768

Purpose

Conversion of C to T in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A and E945K in wild type …

Promoter

maize Ubiquitin-1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-WT

Plasmid

#122690

Purpose

Express His-tagged PKM2 (wild type) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCa-C1

Plasmid

#112264

Purpose

ITRACKalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pVAX1/mTyr-Cre

Plasmid

#99249

Purpose

Expresses Cre recombinase under tyrosinase promoter

Depositor

Christian Blank

Article

Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

Inserts

Cre recombinase
mTyr enh/prom

Tags

Myc

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-B2AR-APEX

Plasmid

#96848

Purpose

For expression of B2AR-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

B2AR-APEX (ADRB2 Human)

Expression

Mammalian

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-AuroraAKD-C-7

Plasmid

#54998

Purpose

Localization: Centrosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

AuroraA KD (AURKA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Kinase Dead - I57V, K162R

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-TGFBR3

Plasmid

#23478

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TGFBR3 (TGFBR3 Human)

Use

Gateway Cloning

Mutation

C44T, 1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (1-145)

Plasmid

#14965

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-145

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
Cdc2-DN

Plasmid

#1887

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-CAMKK1

Plasmid

#23824

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMKK1 (CAMKK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLX304-DCK*-IRES-GFP

Plasmid

#176297

Purpose

Expresses DCK* and GFP from the same transcript

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302 OASL-V5 puro

Plasmid

#158643

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OASL.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OASL (OASL Human)

Use

Lentiviral

Tags

V5

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK2 Q207D

Plasmid

#80871

Purpose

mammalian expression of ALK2 Q207D

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q207D

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV KSR1 IRES GFP

Plasmid

#25973

Depositor

Rob Lewis

Article

Kortum et al Mol Cell Biol. 2004 May . 24(10):4407-16

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pDN-D2irC6kwh

Plasmid

#44725

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
mCherry

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

Plasmid

#90012

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag RPS6KA6

Plasmid

#20623

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KA6 (RPS6KA6 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
NeonGreen

Plasmid

#197924

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

NeonGreen

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y576/577F

Plasmid

#50505

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y576/577F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCLXSN(GFP)-HA-Slc1a5 (mouse)

Plasmid

#71458

Purpose

inducible expression fo Slc1a5 in mammalian cells

Depositor

Shao-Cong Sun

Article

Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

Insert

Slc1a5 (Slc1a5 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

A85P, L125F and V384A mutations compared to GenBa…

Promoter

CMV

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pscAAV-Sox2-mStr

Plasmid

#135617

Purpose

Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

P2A-mStrawberry

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS838 TDP43 RRM-GFP

Plasmid

#98249

Purpose

TDP43 RRM-GFP reporter construct

Depositor

Rajat Rohatgi

Article

Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

Insert

TARDBP (TARDBP Human)

Tags

Internal GFP to replace RRM domains

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
ESCRT-I

Plasmid

#17634

Depositor

James Hurley

Article

Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

Insert

Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIN- myc ULK1 K46I

Plasmid

#27627

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Retroviral

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.GFP-KIF13B tail 442-1843

Plasmid

#134623

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 441 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2TK-YAP-Ag

Plasmid

#19063

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
dABE

Plasmid

#124447

Purpose

Expresses dead Cas9 (dCas9)-ABE7.10

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dABE7.10

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3xflag-APEX2-LRRK2

Plasmid

#164622

Purpose

Expresses LRRK2 tagged with APEX2 and 3xFLAG

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

LRRK2 (LRRK2 Human)

Tags

3xFLAG and APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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