We narrowed to 23,671 results for: CRISPR

1. pPRISM-QF2-he1.1:YFP

   Plasmid

   #184819

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QF2-pA-he1.1:YFP
   

   Use

   CRISPR; Backbone for cloning of homology arms (fo…

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ipGL2Cas

   Plasmid

   #210555

   Purpose

   Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   GL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
   

   Expression

   Plant

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDA010.188

   Plasmid

   #206792

   Purpose

   J23113.dCas9

   Depositor

   James Carothers

   Article

   Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

   Insert

   dCas9
   

   Promoter

   J23113

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-dErbB4

   Plasmid

   #197357

   Purpose

   A knock-out vector for dog ErbB4.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-dErbB1

   Plasmid

   #197354

   Purpose

   A knock-out vector for dog ErbB1.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-dErbB2

   Plasmid

   #197355

   Purpose

   A knock-out vector for dog ErbB2.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-dErbB3

   Plasmid

   #197356

   Purpose

   A knock-out vector for dog ErbB3.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPO646

   Plasmid

   #195307

   Purpose

   pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsD of McCAST
   

   Expression

   Bacterial

   Promoter

   Arabinose inducible arapBAD

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORE303N_CEN12Fuzzy3MYB

    Plasmid

    #194440

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYB

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA array targeting CEN1, Fuzzy3MYB
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    MtU6

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORE303N_CEN1

    Plasmid

    #194439

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA targeting CEN1
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    MtU6

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

    Plasmid

    #188975

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

    Depositor

    Sangdun Choi

    Article

    Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

    Insert

    g1 guide RNA
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

    Plasmid

    #188976

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

    Depositor

    Sangdun Choi

    Article

    Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

    Insert

    g2 guide RNA
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMpGE010-sgRNA-Target1 (MpRTN1)

    Plasmid

    #186295

    Purpose

    Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    sgRNA-Target1
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMLS640

    Plasmid

    #188892

    Purpose

    ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9, Cbr-unc-119
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV‐ABEmaxNGX‐NGA

    Plasmid

    #187059

    Purpose

    ABEmax-SpCas9-NGX‐NGA

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    ABEmaxNGX‐NGA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV‐ABEmaxNGX‐NGC

    Plasmid

    #187060

    Purpose

    ABEmax-SpCas9-NGX-NGC

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    ABEmaxNGX‐NGC
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

    Plasmid

    #73542

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

    Plasmid

    #73543

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV‐ABEmaxNGX

    Plasmid

    #187058

    Purpose

    ABEmax-SpCas9-NGX

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    ABEmaxNGX
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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