We narrowed to 123,065 results for: CIL

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pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pIA036

Plasmid

#200425

Purpose

amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Insert

LacI

Tags

bsdronPA

Expression

Bacterial

Promoter

Ppen

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-PGAL-GFP-Cps1

Plasmid

#207030

Purpose

Expression of GFP-Cps1 under GAL promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Cps1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 2

Plasmid

#207608

Purpose

sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

acccccaaacctgactgact

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Tags

None

Expression

Mammalian

Promoter

SV40

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xV5

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xMyc

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

mRuby3

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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