We narrowed to 33,296 results for: REP

1. pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR

   Plasmid

   #211820

   Purpose

   Lentiviral vector expressing a dead GFP adenine base editing reporter

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   dead GFP reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter and SV40 promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

   Plasmid

   #208630

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   Δ1~34,Δ215~284

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

   Plasmid

   #208632

   Purpose

   for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 53~77, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Expression

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

   Plasmid

   #208624

   Purpose

   for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 1~77, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GB1-His-TEV-A7-Strep (2-180)

   Plasmid

   #190067

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

   Depositor

   Lalit Deshmukh

   Article

   Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

   Insert

   Annexin A7 PRD (ANXA7 Human)
   

   Use

   Tags

   GB1-His-TEV and Strep

   Expression

   Bacterial

   Mutation

   PRD only, residues 2-180

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-SMSr-TEV-Twin-Strep

   Plasmid

   #202530

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SAMD8 (SAMD8 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-luciferase-reporter-CDK5RAP2-SVA

   Plasmid

   #202828

   Purpose

   pGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   intronic SVA within CDK5RAP2 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-luciferase-reporter-AGPAT3-SVA

   Plasmid

   #202827

   Purpose

   pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   intronic SVA located in AGPAT3 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-luciferase-reporter-JAM2-SVA

    Plasmid

    #202832

    Purpose

    pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    JAM2 intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-luciferase-reporter-POFUT2-SVA

    Plasmid

    #202831

    Purpose

    pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    POFUT2 intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-luciferase-reporter-HTT-SVA

    Plasmid

    #202833

    Purpose

    pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    HTT intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-luciferase-reporter-CHAF1B-SVA

    Plasmid

    #202829

    Purpose

    pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    CHAF1B intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-SMS2-TEV-Twin-Strep

    Plasmid

    #202529

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS2 (SGMS2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TGA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB1-A11(2-196, G175R)-Strep

    Plasmid

    #196211

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G175R

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-REPORT(PGK/CMV)-GRE

    Plasmid

    #172336

    Purpose

    Gal4 Response element

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLIB-2STREP-HsHelz-GSSG-MBP_AG

    Plasmid

    #148796

    Purpose

    Mammalian Expression of HsHelz

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz (HELZ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pnEA-NpM-HsNanos3_4-20-Y10E-Strep_W

    Plasmid

    #147827

    Purpose

    Bacterial Expression of HsNanos_4-20-Y10E

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos_4-20-Y10E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM-41P-HsXRN1_1174-1649-Strep_AH

    Plasmid

    #148901

    Purpose

    Bacterial Expression of HsXRN1_1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1_1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge