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176,067 results

Showing: 1981 - 2010 of 176067 results

  6. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  9. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    13425-plasmid-map-sequence-id-360003

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  15. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    Villin-mTQ2-P2A-FlpO-ERT2
    Tags
    mTQ2, linked to FlpO through an P2A ribosomal ski…
    Expression
    Mammalian
    Promoter
    Villin
    Available Since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    67278-plasmid-map-sequence-id-115887

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  16. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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  22. mSwAP-In MC2v2 (pKBA135)

    Plasmid
    #254035
    Purpose
    mSwAP-In payload acceptor vector for delivery of large DNA constructs to mammalian cells. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction.
    Depositor
    Jef Boeke
    Article
    Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.
    Insert
    mNeonGree-P2A-FCU1-T2A-NeoR
    Use
    Synthetic Biology; Mouse targeting
    Expression
    Mammalian
    Promoter
    human EF1a
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    254035-plasmid-map-sequence-id-505345

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  23. pLP-TK (pCTC174)

    Plasmid
    #254034
    Purpose
    mSwAP-In/Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-dTK-P2A-HaloTag expression cassette flanked by LoxM/LoxP sites and BsaI homology arms cloning sites. FCU1-counterselectable backbone.
    Depositor
    Jef Boeke
    Article
    Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.
    Insert
    PuroR-dTK-P2A-HaloTag
    Use
    Synthetic Biology; Mouse targeting, recombinase-m…
    Expression
    Mammalian
    Promoter
    human EF1a
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    254034-plasmid-map-sequence-id-508677

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  28. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

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  29. pAAV-Ef1a-mCherry-IRES-Cre (AAV8)

    Viral Prep
    #55632-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a and Ef1a/IRES
    Tags
    mCherry
    Available Since
    Dec. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1981 - 2010 of 176067 results