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Showing: 1441 - 1460 of 7046 results

  4. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dTET1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104413-plasmid-map-sequence-id-204141

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  5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129028-plasmid-map-sequence-id-256131

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  6. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

    Plasmid
    #193348
    Purpose
    tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    TetO mini CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193348-plasmid-map-sequence-id-379819

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  7. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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  10. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid
    #182527
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    7 Copies of TetO Box
    Use
    Tags
    4XFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182527-plasmid-map-sequence-id-358175

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  11. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185370-plasmid-map-sequence-id-363837

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  15. FLAG-FullLength-TET1-CXXC/LLAA

    Plasmid
    #124399
    Purpose
    Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Shaun Cowley
    Article
    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.
    Insert
    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    CXXC zinc finger deletion (aa 585-624) and L897A/…
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124399-plasmid-map-sequence-id-245260

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Showing: 1441 - 1460 of 7046 results