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258,839 results

Showing: 3701 - 3750 of 258839 results

  5. pL99

    Plasmid
    #78577
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    inducible PnitA-NitR system
    Available Since
    May 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78577-plasmid-map-sequence-id-263121

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  9. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  13. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162380-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Tags
    None
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3-GFP-MT2A

    Plasmid
    #11613
    Depositor
    Steven Johnson
    Insert
    metallothionein 2A (MT2A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    11613-plasmid-map-sequence-id-365342

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  16. pAAV_hSyn1_nLightR (AAV9)

    Viral Prep
    #187180-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV_hSyn1_nLightR (#187180). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightR plasmid DNA. Syn-driven expression of red norepinephrine sensor nLightR. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Tommaso Patriarchi
    Article
    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d
    Promoter
    human Synapsin-1
    Tags
    None
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. DHIP1

    Bacterial Strain
    #226851
    Purpose
    Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Bacterial Resistance
    Streptomycin
    Species
    Escherichia coli
    Available Since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only

  24. pCS2+MLS-HyPer7

    Plasmid
    #136470
    Purpose
    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Use
    Tags
    Tandem mitochondrial targeting signal of cytochro…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136470-plasmid-map-sequence-id-271540

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  25. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Article
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Promoter
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101920-plasmid-map-sequence-id-194554

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  32. TFORF0172

    Plasmid
    #142551
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT2 (ONECUT2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    142551-plasmid-map-sequence-id-397438

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  35. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

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  41. pHR-hSyn-EGFP

    Plasmid
    #114215
    Purpose
    Lentiviral vector (control) that targets expression of EGFP to neurons
    Depositor
    Xue Han
    Article
    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral and Mouse Targeting
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
    Promoter
    human synapsin (hSyn)
    Available Since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    114215-plasmid-map-sequence-id-223405

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  47. pHCA/GAL4(1-93).ER.VP16

    Plasmid
    #108216
    Purpose
    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain
    Depositor
    Didier Picard
    Article
    Louvion et al Gene 1993 Sep 6;131(1):129-34.
    Insert
    Gal4 DBD-ERalpha HBD-VP16
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERalpha HBD has G400V mutation
    Promoter
    ADH1
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108216-plasmid-map-sequence-id-208792

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  48. TFORF3532

    Plasmid
    #145008
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LIN28B (LIN28B Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145008-plasmid-map-sequence-id-287138

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  49. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

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  50. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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Showing: 3701 - 3750 of 258839 results