We narrowed to 7,739 results for: ef1a

1. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

   Plasmid

   #51251

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 789,805,817,848,923,956 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCDEF3‐hTIM3 (no tag)

   Plasmid

   #49212

   Purpose

   expresses human Tim3

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (HAVCR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag‐hTim1

   Plasmid

   #49207

   Purpose

   expresses Flag-tagged human Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (HAVCR1 Human)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pENTR-DMD-Donor

   Plasmid

   #60605

   Purpose

   Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   Hygromycin resistant gene
   

   Use

   Knock-in donor vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3F-Strep-mMALT1

   Plasmid

   #33315

   Depositor

   Vanessa Redecke

   Article

   Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

   Insert

   Malt1 (Malt1 Mouse)
   

   Use

   Tags

   3xFLAG and StrepOne

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pWPXL-Klf4

   Plasmid

   #36981

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. p3F-Strep-mBCL10

   Plasmid

   #33314

   Depositor

   Vanessa Redecke

   Article

   Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

   Insert

   Bcl10 (Bcl10 Mouse)
   

   Use

   Tags

   3xFLAG and StrepOne

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

8. Flag‐mTim1 (Bl/6)

   Plasmid

   #49205

   Purpose

   expresses Flag tagged mouse Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (BL/6 allele) (Havcr1 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. JA740

   Plasmid

   #49939

   Purpose

   Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD GFP (TET1 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. p3F-Strep-hMIB2

    Plasmid

    #33313

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB2 (MIB2 Human)
    

    Use

    Tags

    3xFlag and StrepOne

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

    Plasmid

    #51252

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

13. JA524

    Plasmid

    #49938

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD GFP (TET1 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

14. SL537

    Plasmid

    #49945

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. SL536

    Plasmid

    #49942

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. SL534

    Plasmid

    #49940

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-1 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

17. SL535

    Plasmid

    #49941

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-2 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pEF5/FRT/DEST-3xHA/mGli2 P5-6A

    Plasmid

    #51253

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. SL539

    Plasmid

    #49948

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. SL542

    Plasmid

    #49951

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-8 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

21. SL544

    Plasmid

    #49970

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-10 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

22. SL545

    Plasmid

    #49953

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-11 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

23. SL543

    Plasmid

    #49952

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-9 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

24. SL541

    Plasmid

    #49950

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-7 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

25. SL540

    Plasmid

    #49949

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-6 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pEBB-SBP-HA

    Plasmid

    #36100

    Depositor

    Ezra Burstein

    Type

    Empty backbone

    Use

    Tags

    HA and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMVP and pMAGIC Cloning Systems

    Plasmid Kit

    #1000000155

    Purpose

    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.

    Depositor

    Christopher Newgard

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA5FRT-EF-Pdgfralpha-EGFPN

    Plasmid

    #66787

    Purpose

    Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-mScarlet3-CAAX-IRES-Puro

    Plasmid

    #222639

    Purpose

    Live-cell plasma membrane marker

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mScarlet3-CAAX
    

    Use

    Lentiviral

    Tags

    mScarlet3

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

    Plasmid

    #138460

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-KRAB-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge