Skip to main content
Addgene
Showing: 2251 - 2280 of 7088 results

  8. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  9. T2A Split/Link TurboID Kit

    Plasmid Kit
    #1000000249
    Purpose
    The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

    Enlarge

  17. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

    Enlarge

  19. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

    Enlarge

Showing: 2251 - 2280 of 7088 results