179,463 results

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pECFP-DEVDR-Venus

Plasmid

#24537

Depositor

Peter Sorger

Publication

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVDR-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLM-vexGFP-Oct4

Plasmid

#22240

Purpose

2nd generation lentiviral vector

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

vexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FCK-Arch-GFP

Plasmid

#22217

Purpose

Lentiviral expression of Arch-GFP for optogenetic inhibition

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Publication

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pBiFC-bFOSDelataZipVC155

Plasmid

#22014

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosDelataZIPVC155

Tags

HA

Expression

Mammalian

Mutation

deletion of residues 179-193 in bFos(118-211)

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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RNT1-GFP

Plasmid

#17708

Depositor

Hal Dietz

Publication

Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

Insert

rent1 (UPF1 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Expression

Bacterial

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 barr2 HA

Plasmid

#14692

Depositor

Robert Lefkowitz

Publication

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

HA

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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2X PRE TK luc

Plasmid

#11350

Depositor

Donald McDonnell

Publication

Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

Insert

PRE

Use

Luciferase

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Publication

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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rAAV2/MG1.1

Plasmid

#184540

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.1 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A587L, Q588M; inse…

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Publication

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLS-SV40-mP-EGFP

Plasmid

#137724

Purpose

Lentivirus based EGFP expression vector

Depositor

Nadav Ahituv

Publication

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Insert

SV40 enhancer

Use

Lentiviral

Expression

Mammalian

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Publication

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Publication

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Nme2Cas9_AAV

Plasmid

#119924

Purpose

All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

Depositor

Erik Sontheimer

Publication

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

AAV

Tags

NLS-3xHA-NLS and NLS-NLS

Expression

Mammalian

Promoter

U1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pT4-CMV-GFP

Plasmid

#117046

Purpose

Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

Depositor

Wolfgang Uckert

Insert

GFP

Use

Transposon

Promoter

CMV

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR-FUSN-mCh-Cry2olig

Plasmid

#101224

Purpose

FUS(1-214) fused to mCh-Cry2olig

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2olig

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Publication

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pHR_SFFV

Plasmid

#79121

Purpose

Lentiviral vector for constitutive expression of genes (SFFV promoter)

Depositor

Wendell Lim

Publication

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pBAD/His-miRFP670

Plasmid

#79984

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP670

Expression

Bacterial

Promoter

araB

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Publication

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

Plasmid

#71719

Purpose

Expressing a GFP-tagged degron in the soma of C. elegans

Depositor

Abby Dernburg

Publication

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

auxin-responsive protein IAA17 (AXR3 Mustard Weed)

Tags

EmGFP

Expression

Worm

Mutation

minimal degron sequence (71-114aa) with start cod…

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Expression

Bacterial

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Publication

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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ER-mRFP

Plasmid

#62236

Purpose

Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

Depositor

Erik Snapp

Publication

Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

Insert

ER-mRFP

Tags

mRFP1

Expression

Mammalian

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Publication

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Publication

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of TagBFP-Rab5 fusion

Depositor

Gia Voeltz

Publication

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCS2+hSpCas9

Plasmid

#51815

Purpose

Encode hSpCas9 for in vitro transcription with Sp6

Depositor

Masato Kinoshita

Publication

Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

Insert

humanized S.pyogenes Cas9

Use

CRISPR; Medaka, zebrafish

Tags

3xFLAG

Expression

Mammalian

Promoter

SP6

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pTD103luxI_sfGFP

Plasmid

#48885

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Publication

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI, sfGFP, and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Publication

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA5/FRT-HA-hM3D(Gq)

Plasmid

#45547

Purpose

Expresses hM3D (Gq) neuronal activator

Depositor

Bryan Roth

Publication

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM3D (Gq)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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a1Ha-pcDNA3

Plasmid

#45809

Depositor

Edward Perez-Reyes

Publication

Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

Insert

a1Ha (CACNA1H Human)

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLEX_306

Plasmid

#41391

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

Depositor

David Root

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5

Expression

Mammalian

Promoter

PGK

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

Enlarge
pYSEAP

Plasmid

#37326

Depositor

John Schiller

Publication

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Expression

Mammalian

Promoter

EF1a

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pT2/HB

Plasmid

#26557

Depositor

Perry Hackett

Insert

pT2/HB

Expression

Bacterial

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

Enlarge
pBMN YFP-LID

Plasmid

#31767

Depositor

Thomas Wandless

Publication

Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

Insert

YFP-LID (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

Promoter

LTR

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Publication

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pSYFP2-C1

Plasmid

#22878

Depositor

Dorus Gadella

Publication

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2

Expression

Mammalian

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

Enlarge
pLenti CMV/TO RasV12 Puro (w119-1)

Plasmid

#22262

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

Enlarge
TetO-FUW-Klf4

Plasmid

#20322

Depositor

Rudolf Jaenisch

Publication

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
pB18cmBFPAzurite

Plasmid

#14034

Depositor

Patrick Daugherty

Publication

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Insert

Azurite

Use

Yeast/bacteria optimized codons

Tags

6xHis

Expression

Bacterial

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
p181 pK7-HDAC1 (GFP)

Plasmid

#11054

Depositor

Ramesh Shivdasani

Publication

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Tags

GFP

Expression

Mammalian

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
siSlug3

Plasmid

#10905

Depositor

Bob Weinberg

Publication

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

siSlug (SNAI2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

Enlarge
p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Publication

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge

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