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pCFJ104 - Pmyo-3::mCherry::unc-54

Plasmid

#19328

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pCEP4-puroeGFP

Plasmid

#177922

Purpose

Constitutively expresses eGFP with puromycin selection

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 hE47

Plasmid

#16059

Depositor

Robert Benezra

Article

Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

Insert

E47 (TCF3 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMP71-BFP

Plasmid

#141355

Purpose

pMP71 expression vector containing BFP

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

tagBFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHR'-NRSF-CITE-GFP

Plasmid

#21310

Depositor

Jay Nadeau

Article

Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

Insert

neuron restrictive silencer factor (Rest Mouse)

Use

Lentiviral

Tags

GFP (bicistronic with CITE/IRES)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 20, 2009

Availability

Academic Institutions and Nonprofits only

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pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Promoter

EF1alpha

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCol-TGM

Plasmid

#32715

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Type

Empty backbone

Use

Mouse Targeting and RNAi

Promoter

TRE

Available Since

May 1, 2012

Availability

Academic Institutions and Nonprofits only

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pJL43.1 - Pglh-2::MosTase::glh-2utr

Plasmid

#19332

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pglh-2::Mos1 transposase

Expression

Worm

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMF19

Plasmid

#113662

Purpose

Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

Depositor

Miguel Valvano

Article

Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

Insert

wbbL

Promoter

Ptac

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMRS-empty

Plasmid

#220203

Purpose

mini-Tn7 cloning vector - empty backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW-H1 empty vector

Plasmid

#25870

Purpose

3rd generation lentiviral vector

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCI-neo.mCherry-NEDD4

Plasmid

#38316

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

Nedd4 (NEDD4 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLV.SP146.gp91.eGFP.cHS4

Plasmid

#30476

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymScarletI3_T2A_mTurquoise2

Plasmid

#219829

Purpose

equimolar expression of ymScarletI3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymScarletI3_T2A_mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

Plasmid

#231128

Purpose

Expresses xylanase via a cSAT2.0 scheme

Depositor

Xiaofeng Yang

Article

Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

Insert

xylanase

Tags

cSAT2.0

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCE-BiFC-VC155

Plasmid

#22020

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VC155

Tags

HA

Expression

Worm

Mutation

Changed Alanine 206 to Lysine

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pHR-ALFANb-GB1-CAAX-WPRE

Plasmid

#231599

Purpose

Express ALFANb-CAAX

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

ALFANb-CAAX

Use

Lentiviral

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pRho-DsRed

Plasmid

#11156

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

rhodopsin promoter (RHO Bovine)

Tags

DsRed2

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18-GFP

Plasmid

#220495

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

EGFP

Expression

Mammalian

Promoter

SV40

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pESC-MET

Plasmid

#222482

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LYS

Plasmid

#222483

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-PHLPP2

Plasmid

#22403

Depositor

Alexandra Newton

Article

Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

Insert

PHLPP2 (PHLPP2 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pET29b-Rhobin2

Plasmid

#239018

Purpose

Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pABE-dGFP

Plasmid

#226863

Purpose

Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

EGFP

Expression

Mammalian

Mutation

A111V

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pABE-dGFP-gRNA

Plasmid

#226864

Purpose

Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTGMP

Plasmid

#32716

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-AP-Ub

Plasmid

#232577

Purpose

Express Ubiquitin tagged with an Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBx-Spas-sgRNA-Gm

Plasmid

#105232

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FRP2375

Plasmid

#127581

Purpose

Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance gene

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PtetO7.1_Flagtag_8Glycine linker

Use

Synthetic Biology; Pcr based tagging

Tags

Flag tag, 8glycine linker

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
miR-124-5 promoter GFP

Plasmid

#20792

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-124 (mir124-5 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5-AP(-3)-Ub

Plasmid

#232583

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FRP2370

Plasmid

#127576

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FRP2365

Plasmid

#127575

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV.PGK.GFP.miR9T

Plasmid

#115973

Purpose

miR-9 regulated GFP expression

Depositor

Johan Jakobsson

Article

Akerblom et al Nat Commun. 2013;4:1770. doi: 10.1038/nc

Insert

GFPmiR9T

Use

Lentiviral

Tags

GFP

Promoter

PGK

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Expression

Bacterial

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p3XFLAG-MKL2

Plasmid

#27175

Purpose

For mammalian expression of human MKL2 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Selvaraj et al J Biol Chem. 2003 Oct 24. 278(43):41977-

Insert

MKL2 (MRTFB Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
GW1-ARSeNL

Plasmid

#207873

Purpose

Mammalian expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-GFP11-His3MX

Plasmid

#91951

Purpose

Universal C-terminal GFP11 tagging

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Type

Empty backbone

Expression

Yeast

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGN-Oct-1

Plasmid

#53308

Purpose

plasmid contains POU2F1 (Oct-1) gene with HA-tag

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LZRS-GADD45B

Plasmid

#24955

Depositor

Paul Khavari

Article

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

GADD45B (GADD45B Human)

Use

Retroviral

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.423

Plasmid

#203947

Purpose

Level 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

phlF-PphlF

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP709-N1

Plasmid

#79989

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL3287

Plasmid

#139750

Purpose

Base Cas12a CRISPRi plasmid

Depositor

Himadri Pakrasi

Article

Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL44 - Phsp16.48::MosTase::glh-2utr

Plasmid

#19333

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Phsp-16::Mos1 transposase

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2-nMyc-wt_3'UTR

Plasmid

#31858

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge

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