171,702 results
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Plasmid Kit#1000000086PurposeCRISPR-assisted insertion tagging (CRISPaint) plasmid collection for modular integration of large DNA fragments into user-defined genomic locations via a ligase-4-dependent mechanism.DepositorApplicationGenome EditingVector TypeMammalian ExpressionEditing TypeCRISPRAvailable SinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only
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CMV-Hylight
Plasmid#193447PurposeA fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)DepositorInsertHylight Biosensor
ExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREP-NFAT2
Plasmid#11788DepositorInsertNFAT2 (NFATC1 Human)
ExpressionMammalianAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
TagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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nuclear NAD+ biosensor
Plasmid#186789PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleusDepositorInsertnuclear NAD+ biosensor
UseLentiviralExpressionMammalianAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
US0hisB-pyrF-
Bacterial Strain#12614DepositorBacterial ResistanceTetracyclineAvailable SinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR_5x Gal4 UAS
Plasmid#79119PurposeBase vector for cloning Gal4DBDVP64 inducible genesDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
TagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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B-95.ΔAΔfabR
Bacterial Strain#197934PurposeBL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acidsDepositorBacterial ResistanceNoneAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myosin-IIA-GFP
Plasmid#38297DepositorAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
TagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV9)
Viral Prep#105537-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV1 trial size)
Viral Prep#50465-AAV1.TPurposeReady-to-use AAV1 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)
Viral Prep#73178-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.DepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB1H1-Zif268
Plasmid#12612DepositorInsertZif268 in pB1H1 (Egr1 Mouse)
ExpressionBacterialAvailable SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-LentiB3 Transfer Vector
Plasmid#236912PurposeLentiviral packaging plasmid for generation of LgBiT lentiviral particles to transduce HiBiT cell linesDepositorHas ServiceDNATypeEmpty backboneUseLentiviralTagsLgBiTMutationNoneAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAVS-WT
Plasmid#52135PurposeRetroviral vector for expression of human MAVS in mammalian cellsDepositorAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRExpressionMammalianPromoterhSynAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-RdLight1 (AAV9)
Viral Prep#125708-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-RdLight1 (#125708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-RdLight1 plasmid DNA. Synapsin-driven expression of red genetically encoded dopamine sensor RdLight1. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mVenus C1
Plasmid#27794DepositorHas ServiceCloning Grade DNAInsertmVenus C1
ExpressionMammalianAvailable SinceApril 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOT_7-lenti-EFS-SS1-BBz-2A-puro
Plasmid#217990PurposeExpresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.DepositorInsertSS1-BBz CAR
UseLentiviralAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-GFP
Plasmid#86145PurposeLentiviral vector expressing Cas9-GFPDepositorInsertCas9-GFP
UseCRISPR and LentiviralTagsFLAG and GFPExpressionMammalianPromoterEFSAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec16A
Plasmid#36155PurposeExpresses Sec16A as a GFP fusionDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorAvailable SinceDec. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS2056
Plasmid#239837PurposeOMEGAOff + omegaRNA scaffold AAV expressionDepositorInsertDNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
UseAAVTagsHA and SV40 NLSMutationInsertion of REC domain from NbaCas9-1 + E137K/E4…PromoterEFS; human U6Available SinceJuly 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3
Plasmid#113410PurposeExpresses hA3A-BE3 in mammalian cellsDepositorInserthA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRDM1
Plasmid#25249PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDEF3-vhs
Plasmid#131696Purposeexpresses vhs in mammalian cellsDepositorArticleInsertvhs
ExpressionMammalianPromoterCMVAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.Tau2N4R
Plasmid#226396PurposeExpresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Pol
Plasmid#178782PurposeInducible expression of Taq DNA polymerase in E. coliDepositorInsertTaq DNA Polymerase
ExpressionBacterialAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBFP2-C1
Plasmid#22880DepositorInsertSBFP2
ExpressionMammalianAvailable SinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV1)
Viral Prep#104492-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCV019-catP
Plasmid#232825PurposeRandom transposon mutagenesis vector for Bacteroidales species - chloramphenicolDepositorInsertcatP
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
TagseCFP and eYFPExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
Plasmid#203350PurposeTemplate for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertNano Luciferase
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …PromoterT7Available SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Neo DEST (w531-1)
Plasmid#19067Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, NeoDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX317-EGFP
Plasmid#115439PurposeConstitutive lentiviral expressionDepositorInsertEGFP
UseLentiviralTagsV5ExpressionMammalianPromoterEF1aAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE
Plasmid#138147PurposeExpresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP (AAV2)
Viral Prep#55641-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Flp-dependent)Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
Plasmid#31482DepositorInsertTraffic Light Reporter 1.1 (Sce Target) EF Puro
UseLentiviralMutationmCherry has M9S and M16L mutations. This is what …Available SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only