Showing: 141 - 160 of 7892 results
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Plasmid#86872PurposeTo express POLR2G (RPB7) tagged with VENUS at C terminus.DepositorInsertPOLR2G (POLR2G Human)
UseTagsVENUSExpressionMammalianMutationnonePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains3
Plasmid#78459PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2497-2529PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains2
Plasmid#78460PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2264-2315; Q2513R (see deposi…PromoterAvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
HO-pMET-poly-KanMX4-HO
Plasmid#51665Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
YCe1889 HC_Kan_SV40-polyA_p11
Plasmid#100678Purposepart designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertSV40-polyA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-POLR2E-213
Plasmid#117356PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR POLR2E-213
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1AvailabilityAcademic Institutions and Nonprofits only -
P21-sp64 Poly A
Plasmid#59433Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 21
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
P29-sp64 Poly A
Plasmid#59434Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 29
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
P63-sp64 Poly A
Plasmid#59435Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 63
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HIRA-polyA
Plasmid#59781PurposeExpress H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertHIRA
UseTagsExpressionMammalianMutationPromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HIRAmut-polyA
Plasmid#59782PurposeThis Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).DepositorInsertHIRA
UseTagsExpressionMammalianMutationChange from 'GATCGAAGTTTG' to 'GAc…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S433A POLI
Plasmid#131235PurposeMammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S497A POLI
Plasmid#131236PurposeMammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S520A POLI
Plasmid#131237PurposeMammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 141 - 160 of 7892 results