We narrowed to 14,192 results for: cas9

1. pScCas9

   Plasmid

   #117700

   Purpose

   Mammalian expression vector encoding WT ScCas9

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

   Insert

   Streptococcus canis Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiHiFiCas9

   Plasmid

   #164832

   Purpose

   miHiFiCas9 expressing plasmid

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miHiFiCas9 (contains Brex27 at sequence 5031-5138).
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   change R 691 to A

   Promoter

   CMV

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9cur

   Plasmid

   #71538

   Purpose

   Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   Plasmid curing system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKCcas9dO

   Plasmid

   #62552

   Purpose

   High efficiency Streptomyces genome editing by CRISPR/Cas9 system

   Depositor

   Weihong Jiang

   Article

   Huang et al Acta Biochim Biophys Sin (Shanghai). 201

   Inserts

   Scocas9
   sgRNA ()
   act-orf4 homology arm
   

   Use

   CRISPR; Bacterial genome editing

   Expression

   Bacterial

   Promoter

   j23119 and ptipA

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_Cas9d_Plasmid

   Plasmid

   #239445

   Purpose

   Expression plasmid codon-optimized for E. coli

   Depositor

   David Taylor

   Article

   Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

   Insert

   Cas9d
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_ancCas9d_Plasmid

   Plasmid

   #239444

   Purpose

   Expression plasmid codon-optimized for E. coli

   Depositor

   David Taylor

   Article

   Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

   Insert

   ancCas9d
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAdSh.PGK.Cas9

    Plasmid

    #58253

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    Human codon-optimized S. pyogenes Cas9
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S

    Plasmid

    #149638

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, empty

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PtPuc3_diaCas9_sgRNA

    Plasmid

    #109219

    Purpose

    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

    Depositor

    Per Winge

    Article

    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    LHCF1 terminator, LHCF2 promoter, U6 3' regi…

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBR_Cas9_sp1

    Plasmid

    #154851

    Purpose

    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

    Depositor

    John van der Oost

    Article

    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

    Inserts

    Spcas9 gene
    sgRNA scaffold
    

    Use

    CRISPR

    Tags

    6xHis

    Mutation

    Codon harmonized variant for expression in Rhodob…

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nme2Cas9_pCDest

    Plasmid

    #119923

    Purpose

    Nme2Cas9 CMV-driven mammalian expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Tags

    NLS-3xHA-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9CyMutL

    Plasmid

    #166981

    Purpose

    Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    MutL (mutL )
    CymR
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    pCymRC and pLacI

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    pTPGI (galactose+aTc inducible)

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGW_RUBY_EggCas9

    Plasmid

    #225990

    Purpose

    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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