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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,816 results for: nts

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Showing: 141 - 160 of 11816 results

  1. dxCas(3.7)-VPR

    Plasmid
    #108383
    Purpose
    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Article
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Insert
    dxCas(3.7)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108383-plasmid-map-sequence-id-207172

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  9. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

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  10. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62202-plasmid-map-sequence-id-288285

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  11. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid
    #50461
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    HA and mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50461-plasmid-map-sequence-id-182308

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  12. pYD1

    Plasmid
    #73447
    Purpose
    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
    Depositor
    Dane Wittrup
    Article
    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.
    Type
    Empty backbone
    Tags
    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73447-plasmid-map-sequence-id-364550

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  13. pAAV-CAG-NLS-GFP (AAV PHP.eB)

    Viral Prep
    #104061-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    CAG
    Tags
    GFP
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep
    #104491-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 160 of 11816 results