pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)
Plasmid
#223065
Purpose
Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
Depositor
Insert
entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
Use
CRISPR
Tags
NLS(SV40)-3xFLAG
Expression
Bacterial
Mutation
Three regions of SpCas9 encode type IIS restricti…
Promoter
Dual T7
Available Since
April 23, 2025
Availability
Academic Institutions and Nonprofits only
