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pLenti PGK Neo DEST (w531-1)

Plasmid

#19067

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pECFP-Rac1Q61L

Plasmid

#105292

Purpose

Constitutively active Rac 1

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001147786)

Plasmid

#77192

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx1(yeast)

Plasmid

#65004

Purpose

Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1

Tags

roGFP2

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2072

Plasmid

#141905

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX VAMP7 (1-188)

Plasmid

#42315

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)

Tags

GST

Expression

Bacterial

Mutation

Δ181-220

Promoter

Ptac

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
Cytoplasmic cpGFP

Plasmid

#215709

Purpose

Expresses cpGFP in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCK302

Plasmid

#87768

Purpose

As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Inserts

PrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli

Use

Synthetic Biology

Tags

6xHis Tag

Expression

Bacterial

Promoter

PrhaBAD rhamnose-inducible promoter from E. coli …

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBI/Rabaptin-5

Plasmid

#171851

Purpose

inducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.

Depositor

Guangpu Li

Article

Zhu et al PLoS One. 2010 Feb 16;5(2):e9226. doi: 1

Insert

Rabaptin-5 (human)

Expression

Mammalian

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NECTIN4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156755

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NECTIN4 (NECTIN4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPICZc-HsACTB*-thymosinB-8His

Plasmid

#111146

Purpose

Expresses human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration

Tags

Thymosin beta and a His-tag

Expression

Yeast

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2539

Plasmid

#143372

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX5 (SOX5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2542

Plasmid

#144128

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX5 (SOX5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-Keratin14-N-18

Plasmid

#54136

Purpose

Localization: Cytokeratin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Keratin14 (KRT14 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pJTM-T7-mNeongreen-6xHis

Plasmid

#210868

Purpose

Plasmid used for expressing his--tagged mNeongreen for protein purification.

Depositor

Richard Murray

Article

Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

Insert

mNeongreen-6xHistag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b crRNA backbone

Plasmid

#103854

Purpose

For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has Service

Cloning Grade DNA

Insert

U6-BbsI-BbsI-PspCas13b DR-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF0462

Plasmid

#142632

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB5 (CREB5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3325

Plasmid

#144801

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DLX3 (DLX3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7A)-NFIB

Plasmid

#172303

Purpose

In vitro transcription of NFIB

Depositor

Patrik Verstreken

Insert

NFIB (NFIB Human)

Use

Other

Promoter

T7 promoter A-initiating

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-TWIST2 neo

Plasmid

#197936

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Article

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC27A3

Plasmid

#132116

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A3 (SLC27A3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF2558

Plasmid

#144043

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TLX1 (TLX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-Purg1

Plasmid

#39350

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

natMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
MAC_N_SOX15

Plasmid

#167792

Purpose

Expresses SOX15 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

SOX15 (SOX15 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKCE

Plasmid

#23551

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCE (PRKCE Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
CDK2 gRNA (BRDN0001148866)

Plasmid

#77191

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Bacterial Resistance

60 ug/mL of DAP

Species

Escherichia coli

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only