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We narrowed to 27,510 results for: multi

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Showing: 1621 - 1640 of 27510 results

  4. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58693-plasmid-map-sequence-id-492918

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  8. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Publication
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102560-plasmid-map-sequence-id-198595

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  10. tol2-mpeg1-mcherry

    Plasmid
    #58935
    Purpose
    labels macrophages with mcherry
    Depositor
    Anna Huttenlocher
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mpeg1-mcherry
    Promoter
    mpeg1
    Available since
    Sept. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58935-plasmid-map-sequence-id-91295

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  11. pLAMP1-emiRFP670

    Plasmid
    #136570
    Purpose
    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)
    Depositor
    Vladislav Verkhusha
    Publication
    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1-emiRFP670
    Tags
    emiRFP670
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136570-plasmid-map-sequence-id-270818

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  13. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

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  14. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  18. pH2B-miRFP703

    Plasmid
    #80001
    Purpose
    Histone 2B labeling with near-infrared fluorescent protein miRFP703
    Depositor
    Vladislav Verkhusha
    Publication
    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone cluster 1 H2B family member j (H2BC11 Human)
    Tags
    miRFP703
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80001-plasmid-map-sequence-id-157294

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Showing: 1621 - 1640 of 27510 results