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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,529 results for: SUB

Showing: 4101 - 4150 of 9529 results

  1. Ntng2.a-Fc-His

    Plasmid
    #72120
    Purpose
    Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng2.a (Ntng2 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72120-plasmid-map-sequence-id-131306

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  2. Ntng1.b-Fc-His

    Plasmid
    #72111
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.b (Ntng1 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72111-plasmid-map-sequence-id-139922

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  3. Sdk1-AP-His

    Plasmid
    #72008
    Purpose
    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sdk1 (Sdk1 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72008-plasmid-map-sequence-id-135630

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  6. Sdk1-Fc-His

    Plasmid
    #72134
    Purpose
    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sdk1 (Sdk1 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72134-plasmid-map-sequence-id-139915

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  14. pcDNA3.1-CHRFAM7ADelta2bp-mGFP

    Plasmid
    #62636
    Purpose
    mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop
    Depositor
    Henry Lester
    Article
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Insert
    CHRFAM7A (CHRFAM7A Human)
    Use
    Tags
    mEGFP inserted in M3-M4 loop
    Expression
    Mammalian
    Mutation
    there is a 2-bp deletion in exon 6 of CHRFAM7A ca…
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. PLC-TIR-GFP

    Plasmid
    #52736
    Purpose
    Retroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP and PLCd1 Lipid binding
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-85
    Promoter
    Available since
    June 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. Myc-delta/gamma2SIL

    Plasmid
    #119731
    Purpose
    GABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRD/GABRG2 (Gabrg2, Gabrd Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    119731-plasmid-map-sequence-id-229623

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  21. WT MetNI in pBAD

    Plasmid
    #118254
    Purpose
    Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN Escherichia coli)
    MetI (metI Escherichia coli)
    Use
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose Promoter and None
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118254-plasmid-map-sequence-id-228525

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  24. pFREE019Env

    Plasmid
    #137670
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein (env HIV-1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    137670-plasmid-map-sequence-id-273354

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  25. pBT3102

    Plasmid
    #122548
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aqp-1::CemOrange2 (aqp-1 Synthetic, Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122548-plasmid-map-sequence-id-238198

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  29. pBT2999

    Plasmid
    #122535
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a lysosome localized gene (lmp-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_lmp-1::CemOrange2 (lmp-1 Synthetic, Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122535-plasmid-map-sequence-id-238208

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  32. pBT3047

    Plasmid
    #122547
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    Use
    Tags
    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122547-plasmid-map-sequence-id-238211

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  34. Sema6b(S)-Fc-His

    Plasmid
    #72166
    Purpose
    Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sema6b (Sema6b Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72166-plasmid-map-sequence-id-138959

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  40. Ntng2.a-AP-His

    Plasmid
    #71994
    Purpose
    Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng2.a (Ntng2 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71994-plasmid-map-sequence-id-131180

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  42. Ntng1.d-AP-His

    Plasmid
    #71987
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform d protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.d (Ntng1 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71987-plasmid-map-sequence-id-131173

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  47. Ntng1.c-Fc-His

    Plasmid
    #72112
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform c protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.c (Ntng1 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72112-plasmid-map-sequence-id-131298

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  49. pALN148

    Plasmid
    #239800
    Purpose
    Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain and 4 non-synonymous substitutions
    Depositor
    Iain Cheeseman, Ally Nguyen
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mm-CENPT-Rn-Histone Fold Domain-4NS
    Use
    Retroviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    4 nucleotide substitution in rat histone fold dom…
    Promoter
    Moloney Murine Leukemia Virus (MMLV) LTR promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239800-plasmid-map-sequence-id-475928

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Showing: 4101 - 4150 of 9529 results