168,515 results

1. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

   Plasmid

   #182012

   Purpose

   CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   CalR-HaloTag7-P30-SNAP-tag-KDEL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BCJJ125

   Plasmid

   #117515

   Purpose

   Cas9_3 (with intron) Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_3(intron):GCTT:BsaI
   

   Use

   CRISPR

   Tags

   2xFLAG and NLS

   Expression

   Plant

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gq)

   Plasmid

   #230020

   Purpose

   AAV vector expressing anti-mCherry PAGER(Gq) in neurons

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM4D
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

   Plasmid

   #227004

   Purpose

   AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

   Depositor

   Veerle Baekelandt

   Article

   Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

   Insert

   alpha synuclein A53T mutant (SNCA Human, Synthetic)
   

   Use

   AAV

   Mutation

   A53T

   Promoter

   CMVenh synapsin

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE8r-R153del

   Plasmid

   #208296

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-R153del-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-dDIO-MCS--4x6T

   Plasmid

   #196422

   Purpose

   Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV; Dre/rox; astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Kir2.1 K+ channel scFv [N112B/14]

   Plasmid

   #199423

   Purpose

   Mammalian expression of Kir2.1 K+ channel scFv. Derived from hybridoma N112B/14.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targetingKir2.1 K+ channel (Rat) (Kcnj2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GFP-IRES-mSNCA

   Plasmid

   #185714

   Purpose

   AAV expression of GFP and mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-FLuc-WPRE-HGHpA

    Plasmid

    #87951

    Purpose

    Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

    Depositor

    Mark Kay

    Article

    Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-Dopamine D3 receptor [N331/19R]

    Plasmid

    #114488

    Purpose

    Mammalian Expression Plasmid of anti-Dopamine D3 receptor (Human). Derived from hybridoma N331/19.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Dopamine D3 receptor (Homo sapiens) recombinant mouse monoclonal antibody (DRD3 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFE-Rs-FI-prk

    Plasmid

    #162697

    Purpose

    Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. spaeroides Form IC rubisco and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. GBSGFP insulator

    Plasmid

    #121975

    Purpose

    Gli activity reporter, mouse transgenesis

    Depositor

    James Briscoe

    Article

    Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

    Insert

    GBS(long)-GFP-insulator (Gnb5 Mouse)
    

    Use

    Mouse transgenesis

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

15. Anti-Dopamine D2 receptor [N186/29R]

    Plasmid

    #177509

    Purpose

    Mammalian Expression Plasmid of anti-Dopamine D2 receptor (Rat). Derived from hybridoma N186/29.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Dopamine D2 receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Drd2 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJRoC30_Tv9nL

    Plasmid

    #188069

    Purpose

    PROSS-designed high-redox potential laccase from Trametes versicolor

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    PROSS-designed high-redox potential laccase from Trametes versicolor
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    79 PROSS mutations, described in publication

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-syn-axon-jGCaMP8s-WPRE

    Plasmid

    #186043

    Purpose

    jGCaMP8s expression in axons under the control of the synapsin promoter

    Depositor

    Loren Looger

    Insert

    axon-jGCaMP8s
    

    Use

    AAV

    Tags

    GAP43-6xHis

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. RP1B-PP1alpha

    Plasmid

    #51768

    Purpose

    Expresses PP1 alpha residues 7-330

    Depositor

    Wolfgang Peti

    Article

    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

    Insert

    pp1 alpha
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 alpha residues 7-330

    Promoter

    T7-lac

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pFLAG ARMS

    Plasmid

    #24086

    Depositor

    Moses Chao

    Article

    Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

    Insert

    ARMS (Kidins220 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-gRNA-puro

    Plasmid

    #180426

    Purpose

    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    gRNAscr
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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