179,202 results
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Plasmid#104483PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7f
TagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
ExpressionBacterialPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 HA-Sumo1 WT
Plasmid#48966PurposeExpresses HA-Sumo1 WT in mammalian cellsDepositorAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-beta-3
Plasmid#27289DepositorPublicationAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-FlagMLL-pl-ENL(5613)
Plasmid#20873DepositorPublicationAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WT NFAT1
Plasmid#11100DepositorAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-GFP (AAV1)
Viral prep#37825-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WKKD
Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
TagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…Available sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nMagHigh1-EGFP-CAAX
Plasmid#67300PurposeBlue light dependent photoswitching systemDepositorInsertnMagHigh1-EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-O-FUW-Neurod1
Plasmid#30129DepositorAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MIGR1
Plasmid#27490DepositorPublicationTypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEM-3z/601
Plasmid#26656DepositorInsert
ExpressionBacterialAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK7-HA-S6K1-WT
Plasmid#8984DepositorAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)
Viral prep#26973-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti SpBsmBI sgRNA Hygro
Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-dCas9-3XGFP
Plasmid#64107PurposeExpression of Sp dCas9-3XGFP in mammalian cellsDepositorInsertSp dCas9
UseLentiviralTags3XsfGFPExpressionMammalianPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by