We narrowed to 16,660 results for: aga

1. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. H9 F42A pAcGP67a

   Plasmid

   #41810

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   H9 Super-2 (IL2 Human)
   

   Use

   Tags

   8x His

   Expression

   Insect

   Mutation

   Phenylalanine 42 to Alanine

   Promoter

   Polyhedron

   Available since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

5. AAV-CAG-DIO-APEX2NES

   Plasmid

   #79907

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   APEX2NES
   

   Use

   AAV and Cre/Lox

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-puro-N3FLAG-MYB-FL

   Plasmid

   #105596

   Purpose

   retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Full length MYB (mouse) (Myb Mouse)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-TAT-1-101-FLAG

   Plasmid

   #35981

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1 101 amino acids

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMK198 (GAL-OsTIR1)

   Plasmid

   #140655

   Purpose

   GAL-OsTIR1(WT)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(WT)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-mCAMK-RFP

   Plasmid

   #22908

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMK281 (mCherry2-Hygro)

    Plasmid

    #72797

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPRv2-Beclin1

    Plasmid

    #99574

    Purpose

    Expressed Cas9 with sgRNA targeting Beclin1

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting Beclin1 (BECN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAG sHRPb-FKBP-post mGRASP

    Plasmid

    #73146

    Purpose

    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-FKBP-post mGRASP
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GAL FLAG103QDProGFPp303

    Plasmid

    #15587

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. CMV-G-GECO1.0

    Plasmid

    #32447

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ER-ABKAR

    Plasmid

    #61508

    Purpose

    Expression of AMPK biosensor targeted to the endoplasmic reticulum

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    ER targeting sequence from CYP450 2C1 (see commen…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pClneoMyc human NDR1

    Plasmid

    #37023

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    NDR1 (STK38 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SL016: AAV-CAG-FLEX-CheRiff-GFP

    Plasmid

    #124776

    Purpose

    For AAV based expression of CheRiff in Cre-Positive cells

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    CheRiff-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

19. GAL FLAG103QDProGFP p306

    Plasmid

    #15834

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

20. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGTRm

    Plasmid

    #127197

    Purpose

    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA PCR template
    

    Use

    Template plasmid for generation of golden gate pc…

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmyc-GFP-TNRC6A-(1-924)

    Plasmid

    #42003

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-924

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PM-ABKAR

    Plasmid

    #61506

    Purpose

    Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    Lyn kinase sequence (see comments)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCX-NTOM-SpiCee

    Plasmid

    #140902

    Purpose

    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    NTOM-SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GAL FLAG25QDProGFP p306

    Plasmid

    #15831

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pKD4-Nter-APEX2

    Plasmid

    #112868

    Purpose

    Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

    Depositor

    Eric Cascales

    Article

    Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

    Insert

    APEX2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmyc-GFP-TNRC6A-(1-456)

    Plasmid

    #42005

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-456

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PLKO.1-G3BP1-3'UTR

    Plasmid

    #136038

    Purpose

    G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. L1_lacZgRNA-Ck2

    Plasmid

    #136137

    Purpose

    L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GAL FLAG72QDProGFP p306

    Plasmid

    #15833

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only