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We narrowed to 11,159 results for: ENA

Showing: 4201 - 4250 of 11159 results

  1. pCas9-EcRT-GAL-Nat

    Plasmid
    #232090
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232090-plasmid-map-sequence-id-462306

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  2. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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  3. pENTR_L1-YFP-Turbo-L2

    Plasmid
    #127350
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127350-plasmid-map-sequence-id-246303

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  7. pCAG dCas9-KRAB-2A-EGFP

    Plasmid
    #92396
    Purpose
    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    dCas9_KRAB_2A_EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92396-plasmid-map-sequence-id-178234

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  11. pMOD_B2303

    Plasmid
    #91068
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91068-plasmid-map-sequence-id-181874

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  13. pcDNA3.1-OGT_opt

    Plasmid
    #190858
    Purpose
    For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells.
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190858-plasmid-map-sequence-id-379243

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  14. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

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  18. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  23. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid
    #178043
    Purpose
    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178043-plasmid-map-sequence-id-359586

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  30. pNLF1W

    Plasmid
    #136404
    Purpose
    Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    136404-plasmid-map-sequence-id-271260

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  33. pRedCmOcr

    Plasmid
    #176226
    Purpose
    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB and PL
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176226-plasmid-map-sequence-id-347002

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  35. pAAV-hSyn-ChRger1-TS-EYFP

    Plasmid
    #127243
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127243-plasmid-map-sequence-id-247230

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  37. LacI-pLacI-pII pm

    Plasmid
    #218099
    Purpose
    Phagemid that expresses LacI, and pII under the Lac promoter
    Depositor
    Kevin Esvelt
    Insert
    pII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218099-plasmid-map-sequence-id-432443

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  41. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, PGK, and U6
    Available since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  45. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

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  46. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  50. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid
    #127351
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127351-plasmid-map-sequence-id-246289

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Showing: 4201 - 4250 of 11159 results