We narrowed to 4,284 results for: 261

1. Rab23 cds in pGem-T Easy

   Plasmid

   #80542

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   rab23 (rab23 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFOSGE_COF1D12

   Plasmid

   #70857

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Rpn3 (Rpn3 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFOSGE_COF3B07

   Plasmid

   #71040

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   CoRest (CoRest Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pUC19 gene 60 thru gap

   Plasmid

   #49160

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Use

   Tags

   Expression

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19 gene 60 to gap + Cys

   Plasmid

   #49159

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 600
   

   Use

   Tags

   Expression

   Mutation

   Coding gap and downstream sequence have been dele…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS313-prp8-S2197F

   Plasmid

   #89967

   Purpose

   S2197F point mutant

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   S2197F

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS313-prp8-H2387P

   Plasmid

   #89968

   Purpose

   H2387P point mutant

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H2387P

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS313-prp8-R2388G

   Plasmid

   #89969

   Purpose

   R2388G point mutant

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R2388G

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS314-prp16-1

   Plasmid

   #89972

   Purpose

   prp16-1 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-1 allele (Y386D)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS314-prp16-101

    Plasmid

    #89973

    Purpose

    prp16-101 allele in TRP plasmid

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP16 (PRP16 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    prp16-101 allele (G373S)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPHT-PIP2

    Plasmid

    #60937

    Purpose

    Fluorescent FPX sensor for PIP2

    Depositor

    Anne Marie Quinn

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    PIP2 dimerization dependent sensor RA+B monomers
    

    Use

    Tags

    dimerization dependent fluorescent protein monome…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI-neo FlucDM EGFP

    Plasmid

    #90172

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R188Q, R261Q in FLuc

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-kcnk18

    Plasmid

    #209000

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk18 (kcnk18 Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. WT PBRM1- TRIPZ-neo

    Plasmid

    #107406

    Purpose

    Mammalian expression, inducible PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 7MEQ

    Plasmid

    #176412

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    

    Use

    Baculovirus expresssion

    Tags

    8 x His Tag and Honeybee melittin signal

    Expression

    Mutation

    etmprss2_S251D/R252D/Q253D/S254D/R255K

    Promoter

    Polyhedrin

    Available since

    Oct. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. RA-NES

    Plasmid

    #61019

    Purpose

    ddRFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

    Plasmid

    #162037

    Purpose

    Background-free mRNA labeling tripartite superfolder GFP system

    Depositor

    Hye Yoon Park

    Article

    Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

    Insert

    GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MCP-GFP1-10 P2A PCP-GFP11

    Plasmid

    #162038

    Purpose

    Background-free mRNA labeling bipartite superfolder GFP system

    Depositor

    Hye Yoon Park

    Article

    Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

    Insert

    MCP-GFP1-10 P2A PCP-GFP11
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Mouse 3' Hp1a AID GFP PuroR

    Plasmid

    #127897

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

    Depositor

    Mark Groudine

    Insert

    Hp1a (Cbx5 Mouse, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJAF205

    Plasmid

    #60407

    Purpose

    Flag-tagged full length GMAP210/Trip11

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

    Insert

    GMAP210 (Trip11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3.1/HisC-mTAZ

    Plasmid

    #31793

    Depositor

    Michael Yaffe

    Article

    Hong et al Science. 2005 Aug 12;309(5737):1074-8.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His, and Xpre…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mRIP3 GFP wt

    Plasmid

    #41382

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (Ripk3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFRT/TO/FLAG/HA-DEST TNRC6C

    Plasmid

    #19885

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6C (TNRC6C Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p1195-pssAAV.U1a.hNme2Cas9

    Plasmid

    #129534

    Purpose

    AAV vector expressing Nme2Cas9

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    human codon-optimized Nme2Cas9
    

    Use

    AAV

    Tags

    2xNLS and NLS-3xHA-NLS

    Expression

    Mutation

    Promoter

    U1a

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RALA

    Plasmid

    #55657

    Purpose

    expression of mouse RALA

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RALA (Rala Mouse)
    

    Use

    Tags

    GST and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CaM/pIRES2-eGFP

    Plasmid

    #111499

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    calmodulin [Xenopus laevis] (calm2 Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b-CoilR-Cys

    Plasmid

    #181989

    Purpose

    Used to label proteins with the CoilE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    CoilR-Cys
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LLhG-pHG165a E62K

    Plasmid

    #90051

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

    Insert

    LLhG/E62K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E62K

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEF-TAZ-N-Flag

    Plasmid

    #19025

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pX330-NEAT1m_v1

    Plasmid

    #97083

    Purpose

    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1m_v1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mEm-CoilE-Actin

    Plasmid

    #181993

    Purpose

    CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    mEm-CoilE-Actin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX335-NEAT1_IS_v1

    Plasmid

    #97085

    Purpose

    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1_IS_v1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX335-NEAT1_IS_v2

    Plasmid

    #97086

    Purpose

    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1_IS_v2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. psiCHECK SCN1B 3'UTR

    Plasmid

    #19855

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    SCN1B 3'UTR (SCN1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJES103

    Plasmid

    #135410

    Purpose

    E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter

    Depositor

    Miriam Braunstein

    Article

    McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

    Insert

    blaTEM-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E. coli 'blaTEM-1 with M. tuberculosis mpt63…

    Promoter

    hsp60

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge