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Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLLX8
Plasmid#79838PurposeContains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vectorDepositorTypeEmpty backboneUseContains yeast counter selection gene, cyh2 and b…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-014
Plasmid#78702PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHSP70B-CP6-hph
Plasmid#165879PurposeBasic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-sfGFP
Plasmid#162689PurposepFA vector control expressing sfGFPDepositorInsertsfGFP
UseTagsC terminal 6x His tagExpressionBacterialMutationPromoterPL tet promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SMYD2_2
Plasmid#36350DepositorInsertSMYD2 (SMYD2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
psecBFP2
Plasmid#62230Purposeencodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.DepositorInsertsecBFP2
UseTagssecbfp2ExpressionMammalianMutationPromotercmvAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2
Plasmid#133002PurposepFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
peT3a-AqAdk_MVGDH
Plasmid#18092DepositorInsertadenylate kinase (kad A. aeolicus)
UseTags6xHisExpressionBacterialMutationTyr 52 changed to Cys, Val 145 changed to Cys, …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-rbN1 S77A
Plasmid#32945DepositorInsertSLc9a3r1 (SLC9A3R1 rabbit)
UseTagsT7 tag, his, and partial FLAGExpressionMammalianMutationChange Serine77 to AlaninePromoterCMVAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPWCh
Plasmid#124238PurposeGateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterUASpAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 Sox4 mut 3'UTR oligo
Plasmid#26988DepositorInsertSox4 mut UTR oligo (SOX4 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationbp 449-509 of SOX4 3'UTR; mut @ bp 483PromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-011
Plasmid#78699PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SMYD2_3
Plasmid#36351DepositorInsertSMYD2 (SMYD2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p107 1*
Plasmid#32389DepositorInsertp107 promoter (Rbl1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation1st E2F binding site mutation: GCG-->AAAPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-009
Plasmid#78696PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseH
Plasmid#89725PurposeExpresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage siteDepositorInsertPseH (pseH Campylobacter jejuni)
UseTags2xHis Gln 5xHis and TEVExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLL7.0 tgRFP LOV
Plasmid#114463PurposeMammalian expression of tgRFP labelled LOV2DepositorInsertLOV2
UseTagstgRFPExpressionMammalianMutationG401A(G528A), N411E(N538E) and V289I (V416I)PromoterCMVAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoPL
Plasmid#114467PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoPLDepositorInsertBoPL
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx TOM20 mVenus BoLL
Plasmid#114465PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoLLDepositorInsertBoLL
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoLD
Plasmid#114468PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoLDDepositorInsertBoLD
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-rbN1 S77D
Plasmid#32947DepositorInsertslc9a3r1 (SLC9A3R1 rabbit)
UseTagsT7 tag, his, and partial FLAGExpressionMammalianMutationSerine 77 mutated to Aspartic AcidPromoterCMVAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-N1-PDZ1-GAGA
Plasmid#31651DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsHis, S-tagExpressionMammalianMutationchanged P(21)NGYGF changed to H(21)NGAGAPromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-N1-PDZ2-GAGA
Plasmid#32857DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsHis,S-tagExpressionMammalianMutationPDZ2 P(161)NGYGF mutated to H(161)NGAGAPromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-H1-sgRNA-hTZAP
Plasmid#87186PurposeguideRNA targeting exon1 of human TZAPDepositorInsertTZAP (ZBTB48 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterH1Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-TZAPZNF9-11
Plasmid#87184Purposeretroviral stable expression of truncation protein Flag-TZAPZNF9-11DepositorInsertTZAP (ZBTB48 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 6: AIRAP(1-89).GST,V1.pcDNA3
Plasmid#21796DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionMammalianMutationAA 1-89PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 3: m1.9.9E10.pcDNA3
Plasmid#21793DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsmycExpressionMammalianMutationlacks 5'UTRPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
human pirh2-(138-189)
Plasmid#24881DepositorInsertPirh2 (RCHY1 Human)
UseTagsGSTExpressionBacterialMutationContains aa 138-189.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only