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mTagBFP2-ER-5

Plasmid

#55294

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ER (CALR Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-PE2-N

Plasmid

#164909

Purpose

Expresses the N-terminal split-intein fragment of PE2

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

PE2-N

Use

AAV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCK340

Plasmid

#192639

Purpose

Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dSpG has se…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMK025

Plasmid

#236572

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120395

Purpose

pAAV plasmid expressing scrambled shRNA under the U6 promoter and mCherry.3xFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Insert

Scrambled shRNA

Use

AAV and RNAi

Tags

mCherry.3XFLAG

Promoter

U6

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcs2-GFP-IRES-mCherry

Plasmid

#226098

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2962

Plasmid

#144438

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR2C2 (NR2C2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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BCL2alpha-MJK2243

Plasmid

#227690

Purpose

Expresses human BCL2alpha

Depositor

Curt Civin

Article

Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

Insert

human BCL-2alpha (BCL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2

Plasmid

#17999

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CitA-Tq-HR

Plasmid

#241833

Purpose

Mammalian expression vector for CitA-Tq-HR

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

CitA-Tq-HR

Use

AAV

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CitA-Tq-LR

Plasmid

#241832

Purpose

Mammalian expression vector for CitA-Tq-LR (low-range)

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

CitA-Tq-LR

Use

AAV

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-N LMP2A

Plasmid

#37963

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

LMP2A (LMP-2A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP

Plasmid

#235573

Purpose

Dox-inducible expression of control superfolder (sf)GFP fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

superfolder (sf)fGFP

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAG261-His-greenFAST

Plasmid

#160349

Purpose

Expresses His-tagged greenFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-greenFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-natCX

Plasmid

#73893

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

natCX

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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ALIX-mNeonGreen

Plasmid

#191191

Purpose

Full-length ALIX with C-terminal mNeonGreen tag.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (PDCD6IP Synthetic, Human)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-mmA1

Plasmid

#61090

Purpose

expression of fusion protein: MBP-hnRNP A1

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

hnRNP A1 (Hnrnpa1 Mouse)

Tags

H6_MBP

Expression

Bacterial

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hprtSAGFP

Plasmid

#41594

Purpose

In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

Insert

SA-GFP reporter

Use

Mouse Targeting; Dsb repair reporter substrate

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

Plasmid

#176531

Purpose

PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

KGV transcription factor
TagBFP

Use

Piggybac transposase integration vector

Tags

ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

Expression

Mammalian

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGLS_2

Plasmid

#163459

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting GLS (GLS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GRAB_g5-HT3.0

Plasmid

#208723

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

CaMKII

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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LBR-mCherry

Plasmid

#172446

Purpose

Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

lamin B receptor (LBR Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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