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167,212 results

Showing: 6271 - 6300 of 167212 results

  10. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid
    #140983
    Purpose
    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha11-RLuc8 (GNA11 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140983-plasmid-map-sequence-id-300767

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  16. ADORA1-DuET

    Plasmid
    #213173
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA1 (ADORA1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213173-plasmid-map-sequence-id-430741

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  19. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized denOsCas12f1-VPR
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197031-plasmid-map-sequence-id-388973

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  21. LV-AR-C563A-C596A

    Plasmid
    #171220
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C563A-C596A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C563A-C596A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171220-plasmid-map-sequence-id-342449

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  22. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

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  23. LV-AR-C619Y

    Plasmid
    #171222
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C619Y (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C619Y
    Promoter
    EF-1a
    Available Since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171222-plasmid-map-sequence-id-336120

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  24. plex_307-3xflag-ccdb-blasticidin

    Plasmid
    #201123
    Purpose
    pLEX_307 plasmid with N-terminal FLAG tag and blasticidin marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    201123-plasmid-map-sequence-id-396322

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  25. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid
    #221359
    Purpose
    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221359-plasmid-map-sequence-id-436820

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Showing: 6271 - 6300 of 167212 results