168,189 results

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-a1AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ14901

Plasmid

#239276

Purpose

Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PspCas13b-hGAPDH-gRNA1 (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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ARTi

Plasmid

#214064

Purpose

Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-zSpG

Plasmid

#179316

Purpose

In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

Depositor

Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Zebrafish codon-optimized Cas9 variant SpG

Use

CRISPR; In vitro transcription by t3 promoter

Tags

SV40-NLS

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Article

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Insert

NMHC II-C (Myh14 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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LT3REVIR

Plasmid

#111176

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

Plasmid

#203908

Purpose

Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4UAS_dGFP_PGK_iRFP

Expression

Mammalian

Promoter

5xUAS and PGK

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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SIRT7 Flag

Plasmid

#13818

Purpose

Mammalian expression of human SIRT7 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT7 (SIRT7 Human)

Tags

flag

Expression

Mammalian

Mutation

Missing exon 2

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon

Plasmid

#174092

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

mNeonGreen

Use

Gene tagging

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV1A

Plasmid

#224437

Purpose

Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDLTTP insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCKanR-Mu-BsaI

Plasmid

#79769

Purpose

Transposon-propagation plasmid for Mu-BsaI modified transposon

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mu-BsaI

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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RAD21 C-tag CRISPR

Plasmid

#140540

Purpose

RAD21 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAK004

Plasmid

#233641

Purpose

For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.

Depositor

Zak Swartz

Article

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

eSpCas9(1.1) with c-terminal VitelloTagA

Tags

6-His

Expression

Bacterial

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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p2attNG-H11-long

Plasmid

#51545

Purpose

p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

GFP4m

Use

Human targeting

Expression

Mammalian

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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PGL4-4X Mut Renilla

Plasmid

#70040

Purpose

Control plasmid for testing 5'UTR folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

Random sequence matched for GC-content

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCAX APP C99

Plasmid

#30146

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

APP C99 (APP Human)

Expression

Mammalian

Mutation

C-terminal from BACE site of APP

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX-MCS UBC promoter

Plasmid

#128045

Purpose

Lentiviral vector with UBC promoter

Depositor

Oskar Laur

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_1

Plasmid

#164986

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TFAM-mClover

Plasmid

#129574

Purpose

Expression of mClover fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir2-LNS2

Plasmid

#220086

Purpose

C-terminal PA binding domain of Nir2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM1 (PITPNM1 Human)

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 896-1216

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

Plasmid

#195544

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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