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168,189 results

Showing: 10261 - 10290 of 168189 results

  1. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  10. TFORF0922

    Plasmid
    #142207
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR2F1 (NR2F1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142207-plasmid-map-sequence-id-397659

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  17. LEGO-AP1x6-GM55

    Plasmid
    #217415
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    217415-plasmid-map-sequence-id-441062

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  20. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid
    #191391
    Purpose
    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(30-120)
    Use
    CRISPR and Synthetic Biology
    Tags
    mScarlet-I
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191391-plasmid-map-sequence-id-378101

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  26. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

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Showing: 10261 - 10290 of 168189 results