Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

27,469 results

Showing: 5041 - 5060 of 27469 results

  2. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid
    #189752
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189752-plasmid-map-sequence-id-375290

    Enlarge

  4. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

    Enlarge

  7. pCMV-SaPE2

    Plasmid
    #174817
    Purpose
    Mammalian expression of SaCas9 prime editor 2
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaPE2
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174817-plasmid-map-sequence-id-343608

    Enlarge

  8. pcDNA3.1-GGamma2-GFP2

    Plasmid
    #166774
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma2-GFP2 (GNG2 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166774-plasmid-map-sequence-id-330993

    Enlarge

  14. JD690

    Plasmid
    #160397
    Purpose
    Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation
    Depositor
    Jorge Dubcovsky
    Publication
    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    35S::citrus GRF4-GIF1 chimera
    Promoter
    35S
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    160397-plasmid-map-sequence-id-318344

    Enlarge

  16. iSpyMac

    Plasmid
    #155014
    Purpose
    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iSpyMac
    Expression
    Mammalian
    Mutation
    PID grafted onto SpyCas9 N-terminal domain, R221K…
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    155014-plasmid-map-sequence-id-308820

    Enlarge

  17. miniCGBE1 (pBM1219)

    Plasmid
    #140253
    Purpose
    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE4max(R33A)-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, D10A in Cas9
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140253-plasmid-map-sequence-id-291771

    Enlarge

  18. pDEST-SorLA-v1

    Plasmid
    #154892
    Purpose
    Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays
    Depositor
    Johanna Ivaska
    Publication
    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SORL1 (SORL1 Human)
    Tags
    Venus fragment 1 (v1)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154892-plasmid-map-sequence-id-306906

    Enlarge

  19. pCMV-Target-ACEmax

    Plasmid
    #139105
    Purpose
    Mammalian Target-ACEmax expression vector (pSI-625)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139105-plasmid-map-sequence-id-273275

    Enlarge

Showing: 5041 - 5060 of 27469 results