30,260 results

1. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTOX6

   Plasmid

   #127451

   Purpose

   Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   tse2 toxin
   amilCP chromoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   pRha and tac

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMTTH+H1.4

   Plasmid

   #157796

   Purpose

   Expression of human linker histone H1.4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4 (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. repressilator (pZS1-lTlrLLtCL)

   Plasmid

   #26489

   Depositor

   Michael Elowitz

   Article

   Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

   Insert

   LacI, tetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC-Taq

   Plasmid

   #102793

   Purpose

   Expresses Taq DNA polymerase under WT T7 promoter

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Taq DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJE3-E7

   Plasmid

   #214359

   Purpose

   Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

   Depositor

   Ryan Mehl

   Article

   Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

   Inserts

   2nd generation Tet2 "E7" aminoacyl tRNA synthetase
   Lpp promoted M. jannaschii tRNA
   Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
   Orthogonal "D4II" ColE1 origin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AmpR and lpp

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pet28a-His6-Keap1

   Plasmid

   #62454

   Purpose

   His6 tagged Keap1 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. xGoosecoid

   Plasmid

   #24977

   Depositor

   Edward De Robertis

   Article

   Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

   Insert

   Goosecoid (gsc-a Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pBTK1008 (pSL7)

    Plasmid

    #191003

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. XP12 Xbra

    Plasmid

    #16978

    Depositor

    Randall Moon

    Insert

    Xbra (t.S Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pBbA5c-MevT(CO)-MBIS(CO, ispA)

    Plasmid

    #35151

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5,

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

    Plasmid

    #198292

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1030

    Plasmid

    #22067

    Depositor

    Emmanuelle Bouveret

    Article

    Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

    Type

    Empty backbone

    Use

    Tags

    T18-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBHM2617

    Plasmid

    #234496

    Purpose

    NATR-CnU6-empty sgRNA

    Depositor

    Hiten Madhani

    Article

    Nalley et al bioRxiv 2025.02.14.638277

    Insert

    NAT-PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSP1D1-Strep-II

    Plasmid

    #224209

    Purpose

    Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

    Depositor

    Anne Robinson

    Article

    Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

    Insert

    MSP1D1-Strep-II
    

    Use

    Tags

    Strep-II Tag

    Expression

    Bacterial

    Mutation

    Substitution of 7xHis tag found in pMSP1D1 for a …

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-6P-acatenin

    Plasmid

    #186458

    Purpose

    Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(+)-SUMO-A'

    Plasmid

    #209115

    Purpose

    Bacterial expression of CC-GEMS ligand SUMO-A'

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM33_CLIP_CAP_Gly

    Plasmid

    #178470

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLIP_CAP_Gly (CLIP1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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