30,738 results

1. pBAD24-ALFA-TwinStrep

   Plasmid

   #240248

   Purpose

   Arabinose-inducible plasmid with ALFA and Twin-Strep tags

   Depositor

   Krishna Reddy, Olga Boudker

   Article

   Reddy et al Nat Struct Mol Biol (2025).

   Type

   Empty backbone

   Tags

   10x HIS, ALFA, Thrombin, and Twin-Strep

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMVRha

   Plasmid

   #229553

   Purpose

   Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

   Depositor

   Sandy Schmidt

   Article

   Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD063

   Plasmid

   #212916

   Purpose

   Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-eIF4E-TwStrep

   Plasmid

   #208638

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

   Depositor

   Quanfu Ma

   Insert

   human eIF4E (ENST00000450253) (EIF4E Human)
   

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sumo3 PolQM1

   Plasmid

   #78462

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; Q2513R (please see depositor commen…

   Promoter

   T7

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T3-GST-Tev

   Plasmid

   #177142

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   Tev
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDV5 containing Sb-loop

   Plasmid

   #132695

   Purpose

   pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_loop including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD/His-miRFP670nano3

   Plasmid

   #184663

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   araC

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST14-Cys-DogCatcher

    Plasmid

    #171929

    Purpose

    Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    Cys-DogCatcher
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE-Tn5-162

    Plasmid

    #118543

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGIPZ-PD-L1-EGFP

    Plasmid

    #120933

    Purpose

    Express PD-L1-EGFP fusion protein in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

    Insert

    PD-L1-EGFP
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST14-ARL3

    Plasmid

    #67376

    Purpose

    Expression of native hArl3 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pKS1

    Plasmid

    #84079

    Purpose

    Expresses the non-toxic fragment C of tetanus toxin

    Depositor

    Neil Fairweather

    Article

    Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

    Insert

    Tetanus neurotoxin fragment C
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS6c-RFP

    Plasmid

    #35293

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTK-DN4

    Plasmid

    #180285

    Purpose

    KanR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - KanR - CEN6/ARS4
    

    Expression

    Bacterial and Yeast

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS hTSP-5

    Plasmid

    #13002

    Depositor

    Jack Lawler

    Article

    Newton et al Genomics. 1994 Dec . 24(3):435-9.

    Insert

    COMP (COMP Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Tags

    His6-SBP

    Expression

    Bacterial

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pMRE-Tn5-161

    Plasmid

    #118541

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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