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32,069 results

Showing: 4121 - 4160 of 32069 results

  4. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

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  9. AARS_C2_pNIC-Bio3

    Plasmid
    #153047
    Purpose
    Expression construct for producing Avi tagged Alanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    AARS (AARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153047-plasmid-map-sequence-id-304401

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  13. pMycoFos

    Plasmid
    #84577
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.
    Depositor
    Nicholas Coleman
    Article
    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    April 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    84577-plasmid-map-sequence-id-175425

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  14. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  18. pMBEC6

    Plasmid
    #187598
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187598-plasmid-map-sequence-id-367542

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  23. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Kathleen Collins
    Insert
    PP7 coat protein optimized
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Available Since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28174-plasmid-map-sequence-id-13350

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  32. pRelA*

    Plasmid
    #175590
    Purpose
    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Tags
    Fused with a flexible GS linker to mVenus
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175590-plasmid-map-sequence-id-348362

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  34. pJBEI-6410

    Plasmid
    #47049
    Purpose
    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Mutation
    R364S mutation in MK
    Promoter
    PlacUV5
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47049-plasmid-map-sequence-id-496830

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  39. pET28-GG-PUF

    Plasmid
    #52137
    Purpose
    Receiving vector for PUM-HD assembly and expression in E.coli
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Type
    Empty backbone
    Tags
    PUM-HD flanking region 1' and PUM-HD flankin…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    52137-plasmid-map-sequence-id-80978

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  40. pTCF236 HLA-B*40:01

    Plasmid
    #180463
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*40:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180463-plasmid-map-sequence-id-355082

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Showing: 4121 - 4160 of 32069 results