32,126 results
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Plasmid#131649PurposeBacterial expression of ETS gene ELF1DepositorAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only
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YEplac181-RTS1
Plasmid#8626DepositorAvailable SinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_35bpLinker
Plasmid#157790PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_35bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-HyPer3
Plasmid#42132PurposeGenetically encoded H2O2 probe for ratiometric and fluorescence lifetime imagingDepositorInsertHYPER 3
Tags6HisExpressionBacterialPromoterT5Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0-6H-TEV-KRAS G12D
Plasmid#159439PurposeExpresses KRAS G12D in E.coliDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
TagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
TagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
PPP2R5CA
Plasmid#42369PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUM36-MCS2-myc
Plasmid#109379PurposeShuttle vector for high level expression in mycobacteria.DepositorInsertMCS2-Myc
TagsMycExpressionBacterialPromoterKanamycinAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialPromoterT7Available SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pML433
Plasmid#177190PurposeExpresses recombinant HyPro enzyme in E. coliDepositorInsertHyPro enzyme
ExpressionBacterialPromoterT7Available SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag
Plasmid#167269PurposeOverexpression of SNAP-tag in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7045
Plasmid#167216Purposeoverexpression of CueR in E. coli for cell-free copper sensingDepositorInsertCueR
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (NZF1-3, aa 1-178)
Plasmid#66185PurposeExpresses TRABID NZF1-3 (aa 1-178) in E. coliDepositorInsertTRABID (ZRANB1 Human)
TagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-178 of TRABIDPromoterT7Available SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E1D1
Plasmid#161605PurposeBacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formationDepositorInsertMSP1E1D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
DIRAS2
Plasmid#38819PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A
Plasmid#190114PurposeLevel 1 MoClo plasmid for A. baumanniiDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-C_sfYFPTwinStrep
Plasmid#45944Purposeexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequenceDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsPelB export sequence and Twin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
BARD1 26-327 pET28a
Plasmid#12646DepositorInsertBRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
ExpressionBacterialAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7-SnoopLigase
Plasmid#105627PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7DepositorInsertHaloTag7-SnoopLigase
ExpressionBacterialPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mcherry1
Plasmid#87144PurposeConstitutive E. coli mcherry ExpressionDepositorInsertmcherry
ExpressionBacterialPromoterConstitutive proDAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)
Plasmid#223724PurposeExpression of recombinant protein for purificationDepositorInsertFIP200-CTR (RB1CC1 Human)
Tags6xHis-TEV-EGFPExpressionBacterialMutationaa 1429-1591 onlyAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA
Plasmid#222708PurposeExpression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cellsDepositorTagsHA, MBP, and mycExpressionBacterialAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQadd1F
Plasmid#135660PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxExpressionBacterialPromoterpFerAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only