32,662 results
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pGT-Ah6
Plasmid#122573PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
ExpressionBacterialPromoterlac UV5Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
TagsGSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 Wt
Plasmid#29545DepositorAvailable SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
UbcH5B
Plasmid#60443PurposeExpress E2 UbcH5B in E. ColiDepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST1-TOM1-VHS
Plasmid#44423DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c024
Plasmid#173096PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV313
Plasmid#204956PurposeAMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. nigerDepositorInsertsMad7
hph (hygromycin resistance marker)
sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus fumigatus U3 promoter, Aspergillus ni…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSU80
Plasmid#173834PurposeExpresses 80 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bRCC1
ExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in PET28a for protein expression
Plasmid#39297DepositorAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Dp
Plasmid#79159PurposeExpresses the Dp biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Dp
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-MBP-NLS-RfxCas13d-NLS-HA
Plasmid#171586PurposeBacterial expression of NLS-RfxCas13d-NLS-HADepositorInsertHis-MBP-NLS-RfxCas13d-NLS-HA
TagsHAExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIB170
Plasmid#90193PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_v2
Plasmid#183692PurposeBacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)DepositorInsertER Halo(K73T)
ExpressionBacterialMutationK73T in HalotagAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
ExpressionBacterialPromoterP-tetAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-21(+)Tm9D8* TrpB
Plasmid#207517PurposeExpression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serineDepositorInsertTryptophan synthase β-subunit
ExpressionBacterialPromoterT7Available SinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-PUM1-HD
Plasmid#17543DepositorAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB Neo* PB
Plasmid#138413PurposeMinimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; PiggybacExpressionBacterial and MammalianAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa2
Plasmid#78184PurposeExpresses pcDronpa2 in E. coli strainsDepositorInsertpcDronpa2
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023 - NanoLuc
Plasmid#117727PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporterDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
Plasmid#30505DepositorAvailable SinceNov. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 p37
Plasmid#169015PurposeExpresses GST-p37 (human, codon optimized for expression in bacteria)DepositorInsertp37 (UBXN2B Human)
TagsGSTExpressionBacterialMutationsequence was codon optimized for expression in E.…PromotertacAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-array1
Plasmid#140623PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-FRB-PafA
Plasmid#113404PurposeExpresses FRB-PafA in bacteriaDepositorInsertFRB-PafA
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMD14
Plasmid#138654PurposephiOZJ integrating Streptomyces expression vector, intact RDFDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTBX-1
Plasmid#26067DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_N99D
Plasmid#109410PurposeRepA MutantDepositorInsertrepA
TagsRFPExpressionBacterialMutationN99D repA mutation to increase copy numberAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALACE
Plasmid#31853DepositorTypeEmpty backboneUseEscherichia coli-mycobacterium shuttle plasmidTags6xHISExpressionBacterialPromoterAcetamidase operoneAvailable SinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSU313
Plasmid#169695PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
Tags1xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterGPDpAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB
Plasmid#26607DepositorAvailable SinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_79 aka PAS620
Plasmid#71225PurposeReb_1 with RebA(Q91R)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA( G53A, Q91R)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1MycHis/HIP1
Plasmid#29590DepositorAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
TTK
Plasmid#38907PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~P70a-Bottom-T
Plasmid#87314PurposeExpresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.DepositorInsertSplit-Broccoli system
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p4027 GST-ubiquitin
Plasmid#10861DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKB238
Plasmid#170033PurposeEncodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
6379 H10-K218T
Plasmid#1250DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
6380 H10-K620T
Plasmid#1251DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA4
Plasmid#101043PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA4
TagsGSTExpressionBacterialAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5vio-2300
Plasmid#170987PurposeEncodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.DepositorInsertSh-CAST sgRNA 2300 violacein
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterplacIAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCoofy4
Plasmid#43986DepositorTypeEmpty backboneTagsHis6-MBPExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only
