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Bacterial Expression

29,747 results

Showing: 6501 - 6550 of 29747 results

  4. pBFC0625

    Plasmid
    #186691
    Purpose
    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186691-plasmid-map-sequence-id-371386

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  5. 5T5G (SETD8)

    Plasmid
    #101886
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD8 (KMT5A Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101886-plasmid-map-sequence-id-194755

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  7. pJJS006

    Plasmid
    #188329
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mNeonGreen::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188329-plasmid-map-sequence-id-370757

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  8. FCM101

    Plasmid
    #194298
    Purpose
    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
    Depositor
    Shohei Koide
    Article
    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:
    Insert
    FCM101
    Use
    Tags
    His tag, FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    194298-plasmid-map-sequence-id-381074

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  11. pKB223

    Plasmid
    #170013
    Purpose
    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    C18G
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    170013-plasmid-map-sequence-id-335942

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  12. pET20b_Pa-PirA

    Plasmid
    #128956
    Purpose
    expresses Pseudomonas aeruginosa PirA in E. coli
    Depositor
    James Naismith
    Article
    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24
    Insert
    ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
    Use
    Tags
    6xHis and pelB leader
    Expression
    Bacterial
    Mutation
    Signal peptide sequence has been removed
    Promoter
    T7
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128956-plasmid-map-sequence-id-254679

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  18. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  22. pET21-pelB-LaG-6

    Plasmid
    #172741
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-6
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172741-plasmid-map-sequence-id-340175

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  26. pRS413-GAL1-luc*(-SKL)

    Plasmid
    #40234
    Depositor
    Michael Benton
    Insert
    luc*(-SKL)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    GAL1
    Available since
    Oct. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    40234-plasmid-map-sequence-id-51354

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  29. T4 Lysozyme S38D L99A M102Q N144D

    Plasmid
    #18477
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S38D L99A M102Q N144D
    Promoter
    Available since
    Sept. 15, 2008
    Availability
    Academic Institutions and Nonprofits only

  32. pGBK CUP1 Empty

    Plasmid
    #169710
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169710-plasmid-map-sequence-id-335105

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  33. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  39. GAK

    Plasmid
    #39042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAK (GAK Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. pE2n

    Plasmid
    #17456
    Purpose
    modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    3xHA
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17456-plasmid-map-sequence-id-8624

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Showing: 6501 - 6550 of 29747 results