31,059 results
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Plasmid#177149PurposeExpresses kanamycin when ligated in-frameDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only
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pRSETB-roGFP1-R12
Plasmid#83311PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R12
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/N149K/F223R/S202KPromoterT7Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
Tags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianPromoterCMV,&RhamnoseAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU80
Plasmid#173834PurposeExpresses 80 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bRCC1
ExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYH78
Plasmid#217386PurposePtrc99A-PAmCherry for bacterial expressionDepositorInsertPAmCherry
ExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-155
Plasmid#118537PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSSK WT p85 alpha flag
Plasmid#13429DepositorAvailable SinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28-3'TwStrep
Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneTagsTwin StrepExpressionBacterialPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESK22
Plasmid#64857Purposeexpression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replicationDepositorTypeEmpty backboneExpressionBacterialPromoterPtacAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTR(hCc)
Plasmid#61026PurposeExpresses horse holocytochrome c in E. coliDepositorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-PATagRFP
Plasmid#31945DepositorInsertPATagRFP
TagsHisExpressionBacterialMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoteraraBAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
ExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available SinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA.3.1.ADRB2-NP
Plasmid#134376PurposeNanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.DepositorInsertADRB2-NP (ADRB2 Human)
TagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianPromoterT7Available SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PIK3C2GA
Plasmid#42451PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR2P_P474S_P493N
Plasmid#198695PurposeExpression of human FXR2 protein in E. coliDepositorInsertFXR2P (FXR2 Human)
TagsMBPExpressionBacterialMutationChanged proline 474 to serine and proline 493 asp…PromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAT2B
Plasmid#34795DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
KRASB
Plasmid#25153PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pReporter_4
Plasmid#60565PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_4
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mCherry-FLAG:cat
Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
ExpressionBacterialMutationNAAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-StefinA
Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
tUI-HA
Plasmid#122662PurposeExpress tUI-HA protein in E. coliDepositorInserttUI-HA
TagsHA-tag and His-tagExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-EXO1b
Plasmid#68267PurposeBacterial expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationb form, codons 1-846. Please see depositor commen…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
TagsHis tagExpressionBacterialAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRBV6-5
Plasmid#123425Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535VA
Plasmid#46887PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…ExpressionBacterialPromoterPnisAAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR3C2_Hormone-recep
Plasmid#109848PurposeProtein expression and purification of NR3C2_Hormone-recepDepositorInsertNR3C2_Hormone-recep (NR3C2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialPromoterT7Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-3nmt1
Plasmid#105162PurposeYeast gene targetingDepositorInserthphMX6-3nmt1
Tags3nmt1ExpressionBacterial and YeastPromoter3nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His6-ODC-Ctag
Plasmid#163614PurposeExpresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.DepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1185
Plasmid#119255Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
mT-Sapphire-pBAD
Plasmid#54571PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 511DepositorTypeEmpty backboneTags6xHis and mT-SapphireExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pA5c-tesA-vhb50-8fadR
Plasmid#86601Purposeexpression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoterDepositorInsertstesA
Vhb50
fadR
ExpressionBacterialPromoterLacUV5 and pBADAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS6k-RFP
Plasmid#35294DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only