32,167 results
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Plasmid#111619PurposeeCFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-ecfp
ExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-IS186
Plasmid#175581PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
ExpressionBacterialMutationNOAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry-blind
Plasmid#221181PurposecdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)DepositorInsertcdGreen-Matry(blind)
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive native promotersAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
ExpressionBacterialPromoterT7Available SinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-ATG3
Plasmid#202777PurposeATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stopDepositorAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AsnRS
Plasmid#124105PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWUR 1+2 tetO mut89
Plasmid#80102PurposeEncodes a set of wild-type Cas1+2 and a mutant set of Cas1+2DepositorExpressionBacterialMutationQ24H, P202Q, G241D, E276D, L297QAvailable SinceDec. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
KAT2A
Plasmid#38914PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pThermeleon
Plasmid#86120PurposeBandpass temperature-gated expression of mWasabi and mCherry reportersDepositorInsertsTlpA
mWasabi
TcI38
mCherry
cI
Tags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…PromoterpR/pL, pR/pL, pLacI, and pTlpAAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
ExpressionBacterialAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
Plasmid#174516PurposeRecoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.DepositorInsertsPylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon
ExpressionBacterialMutationCUA anticodon, M.alvus Pyl tRNA with CGA anticodo…PromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
ExpressionBacterialAvailable SinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGEX3X-GST-PTEN
Plasmid#20743DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induci…ExpressionBacterialPromoterCOtetR-PIPL12Available SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPfetFn-Target_v2
Plasmid#199564PurposegRNA expression for FnCas12a in E. coli and clostridiaDepositorInsertSacB
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPthl_fUAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorInsertLOV I427T
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
NSUN2
Plasmid#188059PurposeBacterial expression plasmid for human NSUN2; for activity testingDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFV2-HA
Plasmid#187082PurposeExpression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)DepositorInsertatpBEFHAGDC
Tags6-His beta subunit (atpD) and HA tag subunit a (a…ExpressionBacterialMutationAll Cys codons changed to Ala.Available SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
Tags6xHis and Thrombin cleavage siteExpressionBacterialAvailable SinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only