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31,059 results

Showing: 8551 - 8600 of 31059 results

  1. pXPR-SF61

    Plasmid
    #163916
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277
    Insert
    SF5Phe tRNA synthetase
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163916-plasmid-map-sequence-id-327914

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  6. pCDM4-GLY

    Plasmid
    #49808
    Purpose
    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49808-plasmid-map-sequence-id-74623

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  7. pOPINO_H3

    Plasmid
    #171927
    Purpose
    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_H3
    Tags
    His6
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171927-plasmid-map-sequence-id-338639

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  9. pGEX4T3-Stx3(1-265)

    Plasmid
    #100573
    Purpose
    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.
    Depositor
    Thomas Weimbs
    Insert
    Stx3-1-265 (Stx3 Rat)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    No transmembrane domain, residues 4-264 present.
    Promoter
    tac
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    100573-plasmid-map-sequence-id-192872

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  10. pDE43-MCK

    Plasmid
    #49523
    Purpose
    Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49523-plasmid-map-sequence-id-73915

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  14. pDule2-IBBN (G2)

    Plasmid
    #85501
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85501-plasmid-map-sequence-id-169858

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  16. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  17. pJMP12089

    Plasmid
    #222357
    Purpose
    Tn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Article
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222357-plasmid-map-sequence-id-439068

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  28. pGEX-5X-1 U1-C-FL

    Plasmid
    #129077
    Purpose
    generation of GST-tagged U1-C protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    U1-C (SNRPC Human)
    Expression
    Bacterial
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    129077-plasmid-map-sequence-id-252125

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  35. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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  37. pTXB1-CII

    Plasmid
    #51566
    Purpose
    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cII
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51566-plasmid-map-sequence-id-78825

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  39. pATZ-BFP-HA-GFP11

    Plasmid
    #177721
    Purpose
    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    alpha-1-antitrypsin (SERPINA1 Human)
    Tags
    GFP11, HA, and TagBFP
    Expression
    Bacterial and Mammalian
    Mutation
    E342K (ATZ mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177721-plasmid-map-sequence-id-352621

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  40. pBT3003

    Plasmid
    #122537
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::pisy-1 (pisy-1 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122537-plasmid-map-sequence-id-238215

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  41. HDAC7

    Plasmid
    #25478
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC7 (HDAC7 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. 6NM4

    Plasmid
    #162257
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM9 (PRDM9 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162257-plasmid-map-sequence-id-319826

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  45. FtsZ

    Plasmid
    #74128
    Purpose
    Expresses FtsZ without a peptide
    Depositor
    Lynne Regan
    Insert
    FtsZ
    Tags
    C-terminal linker sequence (G G S G S G S S)
    Expression
    Bacterial
    Promoter
    T5-lac
    Available Since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74128-plasmid-map-sequence-id-468728

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  50. PA-TagRFP-pBad

    Plasmid
    #62793
    Purpose
    Localization: Protein Expression Vector , Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and TagRFP
    Expression
    Bacterial
    Mutation
    none
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8551 - 8600 of 31059 results