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Showing: 8551 - 8600 of 29646 results

  11. pHH0103 FNBP3 WW domain #1

    Plasmid
    #104233
    Purpose
    Bacterial expression of WW domain #1 from FNBP3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    FNBP3 WW domain #1 (PRPF40A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104233-plasmid-map-sequence-id-204803

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  13. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO, native, native (respectively)
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  14. pQCascade-array1

    Plasmid
    #140623
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140623-plasmid-map-sequence-id-306003

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  15. TDRD3 (3PMT)

    Plasmid
    #28274
    Depositor
    Cheryl Arrowsmith
    Insert
    Tudor domain-containing protein 3 (TDRD3 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Tudor domain, residues 553-611.
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. 4LG1

    Plasmid
    #60092
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL21D:JMC072-C04:C227980 (VCPKMT Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pSG020

    Plasmid
    #126503
    Purpose
    Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis
    Depositor
    Ute Romling
    Article
    Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115
    Insert
    disaggregase ClpGgi with 6xHis-tag
    Use
    Tags
    C-terminal 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    126503-plasmid-map-sequence-id-252589

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  25. pES134

    Plasmid
    #127551
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Article
    Wu et al Microbiology (Reading). 2024 Mar;170(3).
    Insert
    F3-flanked spectinomycin resistance cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127551-plasmid-map-sequence-id-251734

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  37. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-177346

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  39. As2

    Plasmid
    #123160
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR-ABS84, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123160-plasmid-map-sequence-id-243993

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  41. pGGD_RT_li_Tq2CFP

    Plasmid
    #136971
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136971-plasmid-map-sequence-id-269106

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  44. Ana GvpC-SpyTag-His in pET28a

    Plasmid
    #85737
    Purpose
    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (fused to SpyTag and His-tagged)
    Use
    Tags
    His-tag and SpyTag
    Expression
    Bacterial
    Mutation
    Added an extra glycine after the methionine at th…
    Promoter
    T7
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    85737-plasmid-map-sequence-id-173841

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  46. pHis17_ParM _1

    Plasmid
    #78201
    Purpose
    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Martin Webb
    Article
    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8
    Insert
    parM (K33A I27C T174A T175N C287A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A I27C T174A T175N C287A
    Promoter
    unknown
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    78201-plasmid-map-sequence-id-151697

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  48. 4MVT

    Plasmid
    #60101
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIAS3:YTC008-B10:C225910 (PIAS3 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. pSG66-KcsA-Kv1.3-sfGFP

    Plasmid
    #102457
    Purpose
    Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Insert
    KcsA-Kv1.3
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    102457-plasmid-map-sequence-id-197242

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Showing: 8551 - 8600 of 29646 results