30,749 results

1. pZS2-123

   Plasmid

   #26598

   Depositor

   Michael Elowitz

   Article

   Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

   Insert

   Synthetic
   

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21a(+)/MBP-His-PINK1

   Plasmid

   #38005

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1.Gα12-LgB115

   Plasmid

   #134363

   Purpose

   Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα12-LgB115 (GNA12 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDULE-ABK

   Plasmid

   #49086

   Purpose

   A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

   Depositor

   Peter Schultz

   Article

   Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

   Inserts

   tRNA synthetase
   tRNA for a photocrosslinking amino acid
   

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   GlnRS and lpp

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB1-SCFP3A

   Plasmid

   #103970

   Purpose

   Plasmid encoding SCFP3A

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   SCFP3A
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, S65T, …

   Promoter

   proC

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS413-GAL1-yEGFP1

   Plasmid

   #40235

   Depositor

   Michael Benton

   Insert

   yEGFP1
   

   Tags

   HIS3MX6

   Expression

   Bacterial

   Promoter

   GAL1

   Available Since

   Oct. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEND-184_Cacnes_mCherry

   Plasmid

   #225610

   Purpose

   Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHybr_r2a>mA-srt-his

   Plasmid

   #124806

   Purpose

   HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgA
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-AspS

   Plasmid

   #159618

   Purpose

   Expression plasmid for AcpS phosphopantetheinyl transferase

   Depositor

   Christopher T Walsh, Jun Yin

   Article

   Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

   Insert

   AcpS
   

   Tags

   6HIS

   Expression

   Bacterial

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-p25

    Plasmid

    #24896

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    p25 (CDK5R1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal truncated version of p35, contains aa …

    Available Since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pMS31: pTwinStrep-SUMO-PmcTnsAB

    Plasmid

    #168164

    Purpose

    PmcTnsAB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsAB
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pProEx-IDE-wt

    Plasmid

    #99014

    Purpose

    Express human Insulin Degrading Enzyme (IDE) in E. coli.

    Depositor

    Wei-Jen Tang

    Article

    Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

    Insert

    insulin degrading enzyme (IDE Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    TRC

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-2TK-14-3-3 eta GST

    Plasmid

    #13277

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 eta (YWHAH Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

14. YPet-pBAD

    Plasmid

    #54860

    Purpose

    Localization: Protein Expression Vector , Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Type

    Empty backbone

    Tags

    6xHis and YPet

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pNHEJ-Cas12a-recX

    Plasmid

    #158714

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pmyc

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrc99S-ssDsbA-CRM197-4xDQNAT

    Plasmid

    #128403

    Purpose

    Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Plasmid EUPp_09

    Plasmid

    #157745

    Purpose

    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

    Depositor

    Kang Zhou

    Article

    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

    Inserts

    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    PgyrA

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PZac2.1 gfaABC1D-Aqp4-eGFP

    Plasmid

    #176747

    Purpose

    Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Aqp4-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCP20T-tsapphire

    Plasmid

    #78464

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    T-Sapphire
    

    Expression

    Bacterial

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM10686

    Plasmid

    #213995

    Purpose

    PrpsM driven expression of mScarlet-I

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mScarlet-I
    

    Expression

    Bacterial

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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