29,740 results

1. p356_DG

   Plasmid

   #234699

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-MBP-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p345_DG

   Plasmid

   #234675

   Purpose

   Golden gate domesticated pET28b with His10-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p347_DG

   Plasmid

   #234677

   Purpose

   Golden gate domesticated pET28b with His10-Thrombin

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Thrombin

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p355_DG

   Plasmid

   #234698

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-Thrombin

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Thrombin

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p352_DG

   Plasmid

   #234682

   Purpose

   Golden gate domesticated pET28b with His10-mNeongreen-bdNEDD8

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-mNeongreen-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p363_DG

   Plasmid

   #234685

   Purpose

   Golden gate domesticated pET28b with His10-bdNEDD8 and Sortase

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-bdNEDD8 and Sortase

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p353_DG

   Plasmid

   #234696

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRecE564T

   Plasmid

   #226825

   Purpose

   Expressing RecE*T controled by Ptrc promoter

   Depositor

   Sheng Yang

   Article

   Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

   Insert

   recE*T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pISFba1

   Plasmid

   #226820

   Purpose

   Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

   Depositor

   Sheng Yang

   Article

   Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

   Insert

   ISFba1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pISFba1reRNA

    Plasmid

    #226822

    Purpose

    Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1 none target guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-51b(+)-cpHaloTag141/145

    Plasmid

    #194660

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag141/145
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-51b(+)-cpHaloTag154/156

    Plasmid

    #194661

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag154/156
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pELxG4

    Plasmid

    #172570

    Purpose

    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LuxR-GFPmut3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTHSSe_43

    Plasmid

    #109241

    Purpose

    pSC101 var1 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET51b(+)_H-Luc

    Plasmid

    #113924

    Purpose

    Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST-Cdk5

    Plasmid

    #24894

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYH78

    Plasmid

    #217386

    Purpose

    Ptrc99A-PAmCherry for bacterial expression

    Depositor

    Anthony Vecchiarelli

    Article

    Hoang et al bioRxiv [Preprint]. 2023 Mar 23:2023.03.

    Insert

    PAmCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC-BETAipi-ispA/dxs

    Plasmid

    #178302

    Purpose

    Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    ispA
    dxs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge