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32,156 results

Showing: 5221 - 5250 of 32156 results

  10. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

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  11. PKMYT1

    Plasmid
    #39061
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKMYT1 (PKMYT1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. p404ADH1

    Plasmid
    #15970
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15970-plasmid-map-sequence-id-8455

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  21. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  22. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

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  23. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid
    #176573
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176573-plasmid-map-sequence-id-348271

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  25. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  30. pBHM2404

    Plasmid
    #173441
    Purpose
    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    mNeonGreen-CBP-2XFLAG-NAT1
    Tags
    CBP-2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    173441-plasmid-map-sequence-id-341226

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Showing: 5221 - 5250 of 32156 results