30,749 results

1. pMB1-spect-PthrC3_8-eGFP

   Plasmid

   #107411

   Purpose

   This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC3_8

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCV020-tetQ

   Plasmid

   #232826

   Purpose

   Random transposon mutagenesis vector for Bacteroidales species - tetracycline

   Depositor

   Athanasios Typas

   Insert

   tetQ
   

   Expression

   Bacterial

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAL-rfp_T

   Plasmid

   #74075

   Purpose

   pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA rfp (T)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-HPII

   Plasmid

   #105839

   Purpose

   expresses E. coli HPII catalase with N-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   hydroperoxidase II catalase
   

   Tags

   6x his tag

   Expression

   Bacterial

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNA2.0-6H-TEV-KRAS G12D

   Plasmid

   #159439

   Purpose

   Expresses KRAS G12D in E.coli

   Depositor

   Kenneth Westover

   Article

   Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

   Insert

   KRAS G12D (KRAS Human)
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL1-eBFP2

   Plasmid

   #102640

   Purpose

   In vitro expression of eBFP2 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   eBFP2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rb-379-792-pGex2TK

   Plasmid

   #21748

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   Rb (RB1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-792 of wt Rb …

   Available Since

   Aug. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

   Plasmid

   #159697

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. phage UbiC G3BP1-SNAP

    Plasmid

    #119949

    Purpose

    Lentiviral vector expressing G3BP1-SNAP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MazF-WT

    Plasmid

    #155202

    Purpose

    To express recombinant MazF protein.

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Insert

    MazF
    

    Expression

    Bacterial

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc E. coli, Fly)
    

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Available Since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetO

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-26b-NbVHH05-HA-His

    Plasmid

    #171567

    Purpose

    Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-HA-His (UBE2J1 Human)
    

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCP30T-mKo1

    Plasmid

    #78477

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    mKO1
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MYST3

    Plasmid

    #25181

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MYST3_13 (KAT6A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains catalytic region ( residues 497-780).

    Available Since

    Oct. 25, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pET26b_AT118-L-V5-His

    Plasmid

    #222848

    Purpose

    E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Tags

    V5-His

    Expression

    Bacterial

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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