32,126 results
-
Plasmid#13396DepositorAvailable SinceFeb. 17, 2009AvailabilityAcademic Institutions and Nonprofits only
-
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP713-HO1
Plasmid#197255PurposeExpresses the protein of miRFP713 and HO1 in bacteriaDepositorInsertmiRFP713
ExpressionBacterialAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CBP-HAT-GST
Plasmid#21093DepositorAvailable SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
B4-4
Plasmid#111478Purposetranslational release factor 1DepositorInserttranslational release factor 1
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT2
Plasmid#34650DepositorAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-ACPs
Plasmid#141140PurposeExpression of holo-[acyl-carrier-protein] synthaseDepositorInsertacpS
TagsMBPExpressionBacterialAvailable SinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRedCm
Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
Tags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV297
Plasmid#204820PurposeAMA1 plasmid with amdS selection markerDepositorInsertamdS
UseFungal expressionExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans trpC promoterAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3
Plasmid#197472PurposeBacterial Expression of human ATG3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-GFP_ColE1
Plasmid#171649PurposeQUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-0-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF_p15A
Plasmid#171651PurposeExpresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.DepositorInsertQF-T7 Stop
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-GFP_ColE1
Plasmid#171653PurposeQUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-5-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-10-T7-GFP_ColE1
Plasmid#171654PurposeQUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-10-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-15-T7-GFP_ColE1
Plasmid#171655PurposeQUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-15-T7
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PARP15-c021
Plasmid#173102PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
TagsmCeruleanExpressionBacterialMutationG287-N470, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTmpGm
Plasmid#176229PurposeA template plasmid for amplification of the cI-hok-aacC4 cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, aacC4
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW357b
Plasmid#227930PurposeLow-stringency accessory plasmid for selectionDepositorInsertsgIII (recoded)
T7c
ExpressionBacterialMutationR632SAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
ExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 and CJ8421_04975 mRNA
Plasmid#194210PurposeExpresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2DepositorInsertCJ8421_04975 mRNA and CJ8421_04975 Rptr2
ExpressionBacterialAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyExpressionBacterialPromoterpLlacO-1 for LuxR, pLux for GFPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2
Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4028 GST-E6AP
Plasmid#8655DepositorAvailable SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STMN1
Plasmid#31326DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
RNF146-c005
Plasmid#173091PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP5
Plasmid#160088PurposeBacterial expression of Tn5-APEX2 fusion protein with linker GSGAGADepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 WT
Plasmid#8594DepositorAvailable SinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pQE32-pr-mEosFP (mEosFP-V69T)
Plasmid#99213PurposeBacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).DepositorInsertpr-mEosFP
ExpressionBacterialMutationmonomeric variant with mutation V69T for primed c…PromoterT5Available SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only