32,157 results
-
Plasmid#171320PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed.T1-SKL fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT1-proD-mCherry
Plasmid#65823PurposeConst. mCherry expression , proD promoter, E. coli ExpressionDepositorInsertmCherry
ExpressionBacterialPromoterproD E . coli Constitutive PromoterAvailable SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH4
Plasmid#71713PurposeMembrane scaffold protein (MSP), helix 4 deletionDepositorAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM534
Plasmid#112530PurposepZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanRDepositorInsertluxCDABE
ExpressionBacterialPromoterPL(HrtO)Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BR1.4
Plasmid#199436Purposeexpresses BAmRed1.4 in bacterial cellsDepositorInsertBAMRed1.4
Tagshis tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CAPNS1_EF-hand_8+EF-hand_5
Plasmid#109995PurposeProtein expression and purification of CAPNS1_EF-hand_8+EF-hand_5DepositorInsertCAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_2
Plasmid#103910PurposeProtein expression and purification of ZO1 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-166
Plasmid#118578PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_MBP_RBFOX2
Plasmid#224292PurposeMBP-RBFOX2 protein purification from bacteriaDepositorInsertRNA binding fox-1 homolog 2 (RBFOX2 Human)
Tags6xHis and MBPExpressionBacterialPromoterT7 promoterAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_PflaB-mCherryBb
Plasmid#118243PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
ExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1JL1
Plasmid#234206PurposeHeterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRS-empty
Plasmid#220203Purposemini-Tn7 cloning vector - empty backboneDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-11-LECT2-V40
Plasmid#183031PurposeExpression of LECT2 V40 variant protein from T7 promoterDepositorAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK2101
Plasmid#65770PurposeBacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNADepositorInsertmammalian codon-optimized SaCas9, and SaCas9 gRNA
UseCRISPRTagsNLS-3xFlagExpressionBacterialPromoterT7 (x2)Available SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mKOκ-EC18154
Plasmid#137077PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR78
Plasmid#90210PurposeExpresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyADepositorInsertsCcaS
Cph8
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7-His
Plasmid#11825DepositorAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSA-13
Plasmid#17327DepositorInsertstreptavidin
ExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…Available SinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD_TypeIC_Cascade_cas3_invivo
Plasmid#196400Purposeexpresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assayDepositorInsertsCas5c
Cas7c
Cas8c
Cas3c
Tags6xHisExpressionBacterialPromoteraraBADAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT12.G
Plasmid#201000PurposepLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApRDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad-mAmetrine
Plasmid#18083PurposeBacterial expression of mAmetrine fluorescent proteinDepositorInsertmAmetrine fluorescent proteins
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
GST-PR-LBD
Plasmid#89117Purposebacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1DepositorInserthuman progesterone receptor ligand binding domain (PGR Human)
TagsGSTExpressionBacterialMutationcontains Ligand Binding Domain (637-933)PromotertacAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3993 pET ubcH7
Plasmid#8654DepositorInsertUbcH7 (UBE2L3 Human)
ExpressionBacterialAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only