32,671 results
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Plasmid#208816PurposeEncodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cellDepositorInsertFive copies of JUB1 binding site within the synthetic promoter
UseSynthetic BiologyExpressionBacterialPromoterJUB1 TF-responsive promoterAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH72
Plasmid#202487PurposeExpresses the nanobody VHH72 with T7 inductionDepositorInsertnanobody VHH72
Tags6XHisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC
Plasmid#218686PurposeEncodes codon optimized LCC enzyme for bacterial expressionDepositorInsertLCC
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCgVchcrRNA
Plasmid#203815PurposeExpression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertCRISPR(BsaI)
UseCRISPRExpressionBacterialAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB256
Plasmid#170039PurposeEncodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Bac2a
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB18cmBFP1A5
Plasmid#14035DepositorInsertBFP1A5
UseYeast/bacteria optimized codonsTags6xHisExpressionBacterialAvailable SinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-EFG1mt
Plasmid#31076DepositorInsertMature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
TagsHisExpressionBacterialMutationIncludes the coding sequence for the mature form.…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
ExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-AQNAT
Plasmid#198211PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_AQNAT
Tags6x His Tag and AQNAT mutated (non-permissible) gl…ExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-MOOR
Plasmid#198212PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tagDepositorInsertsfGFP_MOOR
Tags6x His Tag and MOOR glycosylation tagExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQacR-Q2
Plasmid#74690PurposeQacR-Q2 gateDepositorInsertPTac-RBSQ2-QacR-PQacR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarG2
Plasmid#112814Purposegateway cloning destination vector for expressing enhancer driven CD4-tdGFPDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZP46 Prickle-2
Plasmid#16896DepositorInsertPrickle-2 (prickle2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
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Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialPromoterpT7Available SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-I539E
Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo2
Plasmid#25102DepositorAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
GAL4-BRD4
Plasmid#21943DepositorAvailable SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFNC-3
Plasmid#233296PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7 and unknownAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneTagsHis6 and Twin-Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP1H-mCf
Plasmid#229984PurposeLIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2H-mCf
Plasmid#229985PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneTagsStrep-tag IIExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP3H-mCf
Plasmid#229987PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDAC7
Plasmid#25478PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEO100c
Plasmid#50550PurposeExpresses sfGFP under the PompC1157 promoterDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAraTMDN
Plasmid#47515PurposeContains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assayDepositorInsertIntegrin alpha2B L980A TM-CYTO
ExpressionBacterialPromoterTrcAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN4037
Plasmid#74703PurposeOutput plasmid: PPhlF-PBetI-YFPDepositorInsertPPhlF-PBetI-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZE31ms2
Plasmid#25860DepositorInsertms2 binding site (x2)
ExpressionBacterialMutationnoneAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-2.01 beta
Plasmid#69591PurposeOT-II TCR beta chain cDNA in pCRIIDepositorInsertTCR beta clone 1.1 (Tcrb Mouse)
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FYN-1/1
Plasmid#91413PurposeProtein expression and purification of human SH3 domain construct FYN-1/1DepositorInsertFYN-1/1 (FYN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR357.24
Plasmid#125109PurposeExpression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)159
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PXWABS62(E11-TEV)
Plasmid#123157PurposeFor TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-tDepositorInsertParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
UseSynthetic BiologyExpressionBacterialPromoterParsR-ABS62, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutH
Plasmid#86609PurposeE. coli MutH expression plasmidDepositorInsertE. coli MutH
Tagshis-tag and ald-tagExpressionBacterialPromoterT7Available SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET-B-evoSpCas9-NLS-6xHis
Plasmid#207390PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-evoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661Q, amino acids 1005-1013…PromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNX32A-c014
Plasmid#175486PurposeProtein expression in bacterial cells. PX domain, S17-M197DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
3DAX
Plasmid#51333DepositorInsert3DAX
ExpressionBacterialMutationTruncation: See CommentsPromoterT7Available SinceFeb. 24, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJKR-O-acuR
Plasmid#62569PurposeAcrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.DepositorInsertCFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
PDLIM4_PDZ_1
Plasmid#103941PurposeProtein expression and purification of RIL BGLII PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBL7004
Plasmid#136943PurposeMalachite green aptamer, T7 promoterDepositorInsertmalachite green aptamer (MGA)
UseSynthetic BiologyExpressionBacterialPromoterpT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BR1.0
Plasmid#199435Purposeexpresses BAmRed1.0 in bacterial cellsDepositorInsertBAMRed
Tagshis tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC SNAP-KDEL
Plasmid#104982PurposeLentiviral vector expressing SNAP-KDEL for ER labelingDepositorInsertSNAP-KDEL
UseLentiviralTagsSecretion signalExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-gfp_pJMP2768
Plasmid#208889PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
ExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
6VAH
Plasmid#162273PurposeBacterial ExpressionDepositorAvailable SinceDec. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEutRwc
Plasmid#216334PurposeAn IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfpDepositorInsertsEutR
Superfolder green fluorescent protein
ExpressionBacterialAvailable SinceDec. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPomyces-AsCas12j-2
Plasmid#191655PurposeAll-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2DepositorInsertAsCas12j-2
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only