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Showing: 9001 - 9050 of 29618 results

  5. pXWJ109HgAmp30E

    Plasmid
    #123149
    Purpose
    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123149-plasmid-map-sequence-id-244017

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  6. pCR8/GW/TOPO PCGF1

    Plasmid
    #69770
    Purpose
    Gateway entry vector for PCGF1
    Depositor
    Gerard Cagney
    Insert
    PCGF1 (PCGF1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pGBW-m4136464

    Plasmid
    #149534
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149534-plasmid-map-sequence-id-307562

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  9. pJH645

    Plasmid
    #172169
    Purpose
    Reporter plasmid for pylBCD variant in pBAD backbone
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Inserted amber codon immediately after initiator …
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172169-plasmid-map-sequence-id-338853

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  10. pEGFP C3-YAP-deltaC

    Plasmid
    #21126
    Depositor
    Marius Sudol
    Article
    Tsutomu Oka & Marius et al
    Insert
    Yes-kinase associated protein (YAP1 Human)
    Use
    Tags
    EGFP-C3
    Expression
    Bacterial and Mammalian
    Mutation
    5 Carboxy-terminal amino acids -FLTWL are deleted
    Promoter
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only

  11. J-HARS-4

    Plasmid
    #166529
    Purpose
    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166529-plasmid-map-sequence-id-331465

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  12. L-SARS-7

    Plasmid
    #166527
    Purpose
    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166527-plasmid-map-sequence-id-329199

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  13. UBH14

    Plasmid
    #28158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    E3 ligase UHRF2 (UHRF2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    SRA Domain (residues 419-648)
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. BAT8 (3RJW)

    Plasmid
    #28125
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BAT8 (EHMT2 Human)
    Use
    Tags
    His-tag with integrated thrombin site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 913-1193.
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. FES

    Plasmid
    #38869
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FES (FES Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pET15-EF4n_mFAP2b

    Plasmid
    #159464
    Purpose
    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF4n_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159464-plasmid-map-sequence-id-316655

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  32. PKM2 (3GQY)

    Plasmid
    #25360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PKM2_1 (PKM Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  45. PHF8

    Plasmid
    #39032
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHF8 (PHF8 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    tet(A)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112887-plasmid-map-sequence-id-219894

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  49. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  50. pGM202T7

    Plasmid
    #69993
    Purpose
    E. coli/Streptomyces expression vector containing the T7-promoter
    Depositor
    Günther Muth
    Article
    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(
    Inserts
    aphII
    tsr
    rep
    sso
    T7 promoter
    dso
    His-Orf
    Use
    Tags
    N-terminal and C-terminal His tag encoding sequen…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69993-plasmid-map-sequence-id-129665

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Showing: 9001 - 9050 of 29618 results