32,771 results
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Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-527
Plasmid#11518PurposeGateway destination vector for bacterial expression; His tagDepositorTypeEmpty backboneUseGateway CloningTags6xHisExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
ExpressionBacterialAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
HP17deltaP8
Plasmid#235447PurposeHelper plasmid for regulated phage transferDepositorInsertCoding part of the M13KO7 helper phage genome, except gene VIII
ExpressionBacterialAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
TagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialPromoterT7Available SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_4xUb
Plasmid#199779PurposeUsed for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)DepositorInsert4X Ubiquitin in Tandem
TagsGSTExpressionBacterialAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZP06C
Plasmid#232322PurposepZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.DepositorInsertmCherry
ExpressionBacterialPromoterrpsJAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD(Cbx7)
Plasmid#82525PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.DepositorInsertChromobox Homolog 7 (CBX7 Human)
TagsGSTExpressionBacterialMutationCD(Cbx7) ; amino acids 1–62Available SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
Tags6X HisExpressionBacterialMutationE602DPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MZB211
Plasmid#228494Purpose10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)DepositorInsertpol
Tags10xHis-SUMO(Smt3)ExpressionBacterialMutationA408SPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBB572
Plasmid#27397DepositorAvailable SinceMarch 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7+-SrtA
Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
TagsHis tagExpressionBacterialPromoterT7Available SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MP6
Plasmid#69669PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-mCh
Plasmid#187841PurposeConstitutive mCherry-expression vector for Fusobacterium nucleatumDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD112
Plasmid#119893PurposepNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YopH (residues 164-468)
Plasmid#79749PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.DepositorInsertYopH (residues 164-468)
ExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-mi3
Plasmid#159995PurposeExpresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccinationDepositorInsertSpyCatcher003-mi3
TagsC-TagExpressionBacterialPromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
ExpressionBacterialAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pnsgRNA
Plasmid#172717PurposeDelivery of guide RNA for prime editingDepositorInsertJ23119-gRNA
ExpressionBacterialAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-MagLOV
Plasmid#219957PurposeBacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2DepositorInsertMagLOV
Tags6xHisExpressionBacterialMutationAsLOV2 C450P L496V Q513K G528K D540MPromoterT7Available SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KH (KanR, HK022)
Plasmid#45983DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
ExpressionBacterialPromoternative phage promotersAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2x/b
Plasmid#111571PurposeExpresses a plasma membrane calcium pump hPMCA2x/bDepositorAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-FBFP
Plasmid#122137PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only