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Showing: 11201 - 11250 of 29646 results

  2. pGEX6P1-DEST-HA

    Plasmid
    #119751
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119751-plasmid-map-sequence-id-234027

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  4. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Roger Tsien, Erik Rodriguez
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  6. TOPOII Pdx1 (DM#260)

    Plasmid
    #15025
    Depositor
    Douglas Melton
    Insert
    Pdx1 (Pdx1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  8. RAMP2

    Plasmid
    #39058
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RAMP2 (RAMP2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. T4 Lysozyme N68C A93C WT*

    Plasmid
    #18375
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N68C A93C WT*
    Promoter
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  15. pCB32

    Plasmid
    #111911
    Purpose
    Expresses gRNA to target Nte1 locus and has HygR marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting Nte1 locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111911-plasmid-map-sequence-id-218313

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  20. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70032-plasmid-map-sequence-id-129213

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  26. ARHGAP11AA

    Plasmid
    #25261
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARHGAP11AA:M1-L253 (ARHGAP11A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-L253
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. NCOR2

    Plasmid
    #40737
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NCOR2 (NCOR2 Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Residues 615 to 685 of the SANT2 domain of Nuclea…
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. pBAD_dGFPmut3-AAV

    Plasmid
    #187379
    Purpose
    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187379-plasmid-map-sequence-id-370386

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  37. pJK408

    Plasmid
    #72422
    Purpose
    Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag
    Depositor
    T. J. Kappock
    Insert
    feruloyl esterase
    Use
    Tags
    His5
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72422-plasmid-map-sequence-id-140429

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  42. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  44. pST50Trc4-STRHISNDHFR

    Plasmid
    #64000
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64000-plasmid-map-sequence-id-177406

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Showing: 11201 - 11250 of 29646 results