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Showing: 11301 - 11350 of 29646 results

  9. pHelper_O

    Plasmid
    #173783
    Purpose
    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    186 Integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    E59G - please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173783-plasmid-map-sequence-id-342367

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  13. pAC1748

    Plasmid
    #127982
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    127982-plasmid-map-sequence-id-254497

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  15. pET3-ARL15-His6

    Plasmid
    #201880
    Purpose
    Used for bacterial expression of human protein ARL15
    Depositor
    Richard Kahn
    Insert
    ARL15 (ARL15 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201880-plasmid-map-sequence-id-403026

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  17. pST50Trc3-HISNDHFR

    Plasmid
    #63976
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63976-plasmid-map-sequence-id-177382

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  18. pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

    Plasmid
    #30185
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    30185-plasmid-map-sequence-id-13873

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  19. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

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  20. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

    Plasmid
    #179901
    Purpose
    Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    antiGFPnanobody-mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179901-plasmid-map-sequence-id-357220

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  22. NARS_pNIC-Bio3

    Plasmid
    #153054
    Purpose
    Expression construct for producing Avi tagged Asparaginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    NARS (NARS Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153054-plasmid-map-sequence-id-304473

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  23. pET28a_6HT-TBP6.7(R49A)

    Plasmid
    #112726
    Purpose
    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.
    Depositor
    Joseph Wedekind
    Article
    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64
    Insert
    6His-TEV-TBP6.7(R49A)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    R49A
    Promoter
    T7
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112726-plasmid-map-sequence-id-216329

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  28. FLJ22313

    Plasmid
    #25321
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh55.009.085 (HERPUD2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pUC57 DYSF, alt exon 1, 5a, 40a

    Plasmid
    #87889
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 5a, 40a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87889-plasmid-map-sequence-id-174216

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  35. Endophilin II Full

    Plasmid
    #47409
    Depositor
    Peter McPherson
    Insert
    Endophilin II
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  39. pET21-10XHis-GST-HRV-Her2-dL5-Her2

    Plasmid
    #85624
    Purpose
    Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)
    Depositor
    Marcel Bruchez
    Insert
    10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Schistosoma japonicum, Human, Synthetic)
    Use
    Tags
    10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    85624-plasmid-map-sequence-id-287943

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  49. pST50Trc2-STRDHFR

    Plasmid
    #63955
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63955-plasmid-map-sequence-id-177361

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  50. eGFP-Silicatein-pET28

    Plasmid
    #198012
    Purpose
    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification
    Depositor
    Bryan Berger
    Article
    Vigil et al Materials Advances 2, 2023
    Insert
    eGFP-silicatein
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198012-plasmid-map-sequence-id-388331

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Showing: 11301 - 11350 of 29646 results