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31,059 results

Showing: 9241 - 9280 of 31059 results

  3. pBT3007

    Plasmid
    #122538
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-5 (rab-5 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122538-plasmid-map-sequence-id-238129

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  6. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid
    #89154
    Purpose
    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli
    Depositor
    Frédéric Allain
    Article
    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n
    Insert
    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    Tags
    Intein and chitin binding domain
    Expression
    Bacterial
    Mutation
    mutations: Cys 250 to Ser, Cys 251 to Ser
    Promoter
    T7
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89154-plasmid-map-sequence-id-175723

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  15. T4 Lysozyme S38D L99A M102Q N144D

    Plasmid
    #18477
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    S38D L99A M102Q N144D
    Available Since
    Sept. 15, 2008
    Availability
    Academic Institutions and Nonprofits only

  17. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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  19. ATAD2

    Plasmid
    #38916
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ATAD2 (ATAD2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38916-plasmid-map-sequence-id-151434

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  20. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pAtCHYb1Δ129N

    Plasmid
    #53367
    Purpose
    Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.
    Insert
    chyB1 (BETA-OHASE 1 Mustard Weed)
    Expression
    Bacterial
    Mutation
    Deleted codons for the first 129 amino acids
    Promoter
    Trc
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    53367-plasmid-map-sequence-id-109672

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  23. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  27. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available Since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

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  28. pGBW-m4046910

    Plasmid
    #145672
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145672-plasmid-map-sequence-id-297836

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  30. 4H4H

    Plasmid
    #51314
    Depositor
    Cheryl Arrowsmith
    Insert
    4H4H (EHMT1 4H4H, Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. AKAP13

    Plasmid
    #73232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKAP13 (AKAP13 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1976-2211
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  39. PTPRK

    Plasmid
    #38968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRK (PTPRK Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 9241 - 9280 of 31059 results