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Showing: 11801 - 11850 of 29656 results

  1. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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  4. pPro29b(+)

    Plasmid
    #25400
    Depositor
    Jay Keasling
    Insert

    Use
    Synthetic Biology
    Tags
    Unknown
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    Available since
    June 21, 2010
    Availability
    Academic Institutions and Nonprofits only
    25400-plasmid-map-sequence-id-11852

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  7. CBS

    Plasmid
    #73238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBS (CBS Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    73238-plasmid-map-sequence-id-150431

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  9. DNMT3B (3QKJ)

    Plasmid
    #32044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3B (DNMT3B Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acids 293-442)
    Promoter
    Available since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. UCK1A

    Plasmid
    #42374
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UCK1A (UCK1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pGEX4T-1-6AlanineHE1

    Plasmid
    #165098
    Purpose
    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1
    Depositor
    Arthur Haas
    Insert
    Uba1 (UBA1 Human)
    Use
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165098-plasmid-map-sequence-id-326697

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  21. pFTK088

    Plasmid
    #171360
    Purpose
    AMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    AMA1 sequence (short), entry vector providing fungal replication
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    171360-plasmid-map-sequence-id-351789

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  22. pPT-PCR C2

    Plasmid
    #141247
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141247-plasmid-map-sequence-id-377468

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  28. pZA-mCherry-R5

    Plasmid
    #106258
    Purpose
    Expresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetO
    Depositor
    Urartu Seker
    Article
    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi
    Insert
    mCherry
    Use
    Tags
    Added silaffin R5 peptide to C terminus of mCherr…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 22, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. ACOT12BA

    Plasmid
    #42395
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACOT12BA (ACOT12 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. pST50Trc4-STRNDHFR

    Plasmid
    #63996
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63996-plasmid-map-sequence-id-177402

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  39. pET-MBP-CfaN-His6

    Plasmid
    #126017
    Purpose
    Bacterial expression of MBP-CfaN fusion protein (with C-terminal His5)
    Depositor
    Thomas Muir
    Insert
    MBP-CfaN-His5
    Use
    Tags
    5xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126017-plasmid-map-sequence-id-248771

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  40. pET-30a(+)-Syk-tSH2

    Plasmid
    #111269
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111269-plasmid-map-sequence-id-215599

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  43. EPHA3 (2GSF)

    Plasmid
    #25136
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    epha3.577.947 (EPHA3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. IBMc071

    Plasmid
    #161943
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161943-plasmid-map-sequence-id-327544

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  46. p406GALS

    Plasmid
    #17422
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    p406GALS (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17422-plasmid-map-sequence-id-8597

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  48. pTL-Bag3-gRNA3

    Plasmid
    #196254
    Purpose
    Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196254-plasmid-map-sequence-id-385944

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  49. pTL-Bag2-gRNA2

    Plasmid
    #196252
    Purpose
    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196252-plasmid-map-sequence-id-385943

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  50. pTL-Bag1-gRNA1

    Plasmid
    #196251
    Purpose
    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196251-plasmid-map-sequence-id-385941

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Showing: 11801 - 11850 of 29656 results