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Showing: 11851 - 11900 of 29656 results

  1. pTL-Bag4-gRNA4

    Plasmid
    #196255
    Purpose
    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196255-plasmid-map-sequence-id-385931

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  7. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Use
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  12. pCLxR6

    Plasmid
    #172557
    Purpose
    Inducible gene expression, pCOLA origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    Nov. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    172557-plasmid-map-sequence-id-346741

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  15. pEV_S

    Plasmid
    #100266
    Purpose
    pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli
    Depositor
    Timothy Lu
    Article
    Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.
    Inserts
    entD (entD Escherichia coli)
    entC
    entE
    entB
    entA
    vibH
    vibF
    cos
    Use
    Synthetic Biology; Expression, pbr322
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100266-plasmid-map-sequence-id-193235

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  19. NUDT22 (NUDT22A-c001)

    Plasmid
    #98230
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT22A (NUDT22 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98230-plasmid-map-sequence-id-193410

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  20. RP1B-PP1beta

    Plasmid
    #51769
    Purpose
    Expresses PP1 beta residues 6-327
    Depositor
    Wolfgang Peti
    Insert
    pp1 beta
    Use
    Tags
    His, TEV protease site, and Thio-6
    Expression
    Bacterial
    Mutation
    Contains PP1 beta residues 6-327
    Promoter
    T7-lac
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pGEX6P1-human LIC1 C-terminal half

    Plasmid
    #74599
    Purpose
    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Tags
    N-terminal GST and Strep II
    Expression
    Bacterial
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    T7
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74599-plasmid-map-sequence-id-145961

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  25. pQCascade-IS3

    Plasmid
    #140626
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140626-plasmid-map-sequence-id-305994

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  26. PBRM1

    Plasmid
    #39103
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. pAL-pyk_T

    Plasmid
    #74070
    Purpose
    pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pyk (T)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. PBC002-A04

    Plasmid
    #58761
    Purpose
    bacterial expression of PDB 4PWY
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PWY (PEX6 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. pE-SUMO CRYGS

    Plasmid
    #80753
    Purpose
    Expression of human gamma S-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80753-plasmid-map-sequence-id-153560

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  46. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

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  49. pBluescript alpha-SSeCKS

    Plasmid
    #61295
    Purpose
    contains rat alpha SSeCKS cDNA
    Depositor
    Irwin Gelman
    Insert
    alpha SSeCKS (Akap12 Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cDNA with 1kb of 3' UTR and 150bp of 5'…
    Promoter
    T7
    Available since
    Dec. 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. pFTK039

    Plasmid
    #171311
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    pyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171311-plasmid-map-sequence-id-350209

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Showing: 11851 - 11900 of 29656 results