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32,684 results

Showing: 11851 - 11900 of 32684 results

  1. RGD-sfGFP-I27-CC

    Plasmid
    #85392
    Purpose
    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85392-plasmid-map-sequence-id-172694

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  4. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

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  6. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  7. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  8. T7RNAP Cyslight A188C

    Plasmid
    #205083
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S N88C
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205083-plasmid-map-sequence-id-413708

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  10. GLS2A-c007

    Plasmid
    #53621
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    GLS2A
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  17. pBV58

    Plasmid
    #204603
    Purpose
    ERG11-3X Minidegron-5X FLAG-HIS3MX6
    Depositor
    Scott Moye-Rowley
    Insert
    Candida glabrata ERG11 (ERG11 )
    Tags
    auxin-inducible degron
    Expression
    Bacterial
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204603-plasmid-map-sequence-id-403443

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  27. pBT3035

    Plasmid
    #122543
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_lmn-1::CemOrange2 (lmn-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122543-plasmid-map-sequence-id-238214

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  28. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Available Since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

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  29. C-BLa 1.1

    Plasmid
    #88997
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.1 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88997-plasmid-map-sequence-id-177504

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  30. Pk-SOD1

    Plasmid
    #25574
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pk-SOD1
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. PAHA

    Plasmid
    #74663
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKU1 (PAH Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  42. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  49. pHflu2

    Plasmid
    #203880
    Purpose
    pHI1 with insertion of ampicillin-resistance gene
    Depositor
    Bogumil Karas
    Insert
    broad-spectrum class A beta-lactamase TEM-116
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203880-plasmid-map-sequence-id-403830

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Showing: 11851 - 11900 of 32684 results