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31,059 results

Showing: 9561 - 9600 of 31059 results

  2. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  3. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  9. pOP3MT

    Plasmid
    #112601
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    Avi - 8His-MBP
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112601-plasmid-map-sequence-id-220215

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  10. RAB14

    Plasmid
    #25519
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB14 (RAB14 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pGBK CUP1 EGFP

    Plasmid
    #170210
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    enhanced green fluorescent protein
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Promoter
    CUP1
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170210-plasmid-map-sequence-id-335104

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  17. pMCSG53_Nsp3_Unique

    Plasmid
    #167258
    Purpose
    Bacterial expression of Nsp3 Macrodomain 2 and Macrodomain 3 (residues 413-676)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 413-676) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167258-plasmid-map-sequence-id-331340

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  22. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  27. HK3

    Plasmid
    #25303
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HK3:R480-R922 (HK3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids R480-R922
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. CBX1

    Plasmid
    #25245
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX1_03 (CBX1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. DinoIII

    Plasmid
    #128100
    Purpose
    Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.
    Depositor
    Senjie Lin
    Article
    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m
    Insert
    RNA complex & upstream/downstream of LHC
    Expression
    Bacterial
    Promoter
    Inserts are potential promoters
    Available Since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    128100-plasmid-map-sequence-id-292693

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  32. pAM5085

    Plasmid
    #85122
    Purpose
    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    Tags
    ECFP
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85122-plasmid-map-sequence-id-180108

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  35. pCR8/GW/TOPO PCGF1

    Plasmid
    #69770
    Purpose
    Gateway entry vector for PCGF1
    Depositor
    Gerard Cagney
    Insert
    PCGF1 (PCGF1 Human)
    Expression
    Bacterial
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. RAB31

    Plasmid
    #25552
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB31 (RAB31 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pFP.R274

    Plasmid
    #138223
    Purpose
    Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138223-plasmid-map-sequence-id-296592

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Showing: 9561 - 9600 of 31059 results